An MspI polymorphism for the HOX2F gene.
An MspI polymorphism for the HOX2F gene.
复制标题
HOX2F 基因的 MspI 多态性。
DOI：
10.1093/nar/19.7.1716
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发表时间：
1991
影响因子：
14.9
通讯作者：
Kidd,KK
中科院分区：
文献类型：
--
作者：
Ogura,T;Castiglione,CM;Pakstis,AJ;Kidd,KK
来源/描述：ph2-1-3Bg2是一个1.2 kb的BgII片段，亚克隆到Bluescrip ks+载体(Stratagene)的BamHI位点。它是从人基因组文库(T.Ogura)中分离到的粘粒克隆CH2-1的一个5.6kb的BamHI片段PH2-1-3。通过将粘粒CH2-1的限制性内切酶图谱与Acampora等人的限制性内切酶图谱进行同源性比较，克隆PH2-1-3含有HOX2Fhomeobox结构域。通过测序证实了这一点。多态：在PH2-1-3Bg2中，MspI检测到两个等位基因多态，等位基因条带分别位于2.0kb或1.8kb。在1.0kb处有一条微弱的恒定带。频率：等位基因频率基于四个欧洲混血大家庭的49个无关个体和63个无关的欧洲CEPH父母(即排除委内瑞拉人和相关父母)。
Source/Description: pH2-1-3Bg2 is a 1.2 kb BgII fragment subcloned into the BamHI site of Bluescript ks+ vector (Stratagene). It was derived from pH2-1-3, a 5.6 kb BamHI fragment from the cosmid clone cH2-1 isolated from a human genomic library (T. Ogura) using the HOX2A clone pBS1 (T. Mild). The clone pH2-1-3 contains the HOX2Fhomeobox domain by homology of the restriction maps of cosmid cH2-1 with those of Acampora et aL (1). This was confirmed by sequencing thishomeobox.Polymorphism: With pH2-1-3Bg2 MspI detects a two allele polymorphism with allelic bands at either 2.0 kb or 1.8 kb. There is a faint constant band at 1.0 kb. Frequency: Allele frequencies are based on 49 unrelated individuals in four large families of mixed European ancestry and 63 ofthe unrelated European CEPH parents (ie Venezuelans and related parents excluded).