Determination of binding kinetics using whispering gallery mode microcavities
Determination of binding kinetics using whispering gallery mode microcavities
复制标题
DOI：
10.1063/1.3634023
复制
发表时间：
2011-09-05
影响因子：
4
通讯作者：
Armani, Andrea M.
中科院分区：
文献类型：
--
作者：
Soteropulos, Carol E.;Hunt, Heather K.;Armani, Andrea M.
二氧化硅光学微腔传感器在结合对的动力学评估方面显示出巨大的前景，这对于理解生物分子相互作用至关重要。在这里，我们开发并展示了一个基于生物共轭二氧化硅微球谐振器的新型平台，用于研究生物素-链霉亲和素系统的结合动力学。我们表征了光学性能，验证了生物素与表面的共价连接，并进行了链霉亲和素检测实验。我们对检测数据进行了初步动力学分析，显示了回音壁模式谐振器在确定结合对解离常数方面的潜力，这与之前发布的值非常一致。 (C) 2011 年美国物理研究所。 [号码：10.1063/1.3634023]
Silica optical microcavity sensors show great promise in the kinetic evaluation of binding pairs, fundamental in understanding biomolecular interactions. Here, we develop and demonstrate a novel platform, based on bioconjugated silica microsphere resonators, to study the binding kinetics of the biotin-streptavidin system. We characterize the optical performance, verify the covalent attachment of biotin to the surface, and perform streptavidin detection experiments. We perform preliminary kinetic analysis of the detection data which shows the potential of whispering gallery mode resonators in the determination of the dissociation constant of the binding pair, which is in good agreement with previously published values. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3634023]