Distinct patterns of myogenic motor activity identified in isolated human distal colon with high-resolution manometry.
Distinct patterns of myogenic motor activity identified in isolated human distal colon with high-resolution manometry.
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DOI：
10.1111/nmo.13871
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发表时间：
2020-10
影响因子：
3.5
通讯作者：
Dinning PG
中科院分区：
文献类型：
--
作者：
Rosli RM;Heitmann PT;Kumar R;Hibberd TJ;Costa M;Wiklendt L;Wattchow DA;Arkwright J;de Fontgalland D;Brookes SJH;Spencer NJ;Dinning PG
结肠高分辨率测压（HRM）已被用于揭示离散的、传播的结肠运动模式。为了帮助确定这些模式背后的机制，我们使用HRM记录人类远端结肠离体收缩活动。将手术切除的下行结肠（n = 30）或乙状结肠（n = 4）段浸泡在含氧Krebs溶液中，温度36℃（n = 34; 16）（女性；67.6±12.4岁；长24.7±3.5cm;）用HRM导管记录收缩力。基线记录30分钟后，应用0.3mM利多卡因和/或1mM六甲溴铵。在给药前后分别用电场刺激（EFS; 10Hz, 0.5ms, 50V，持续时间5s）激活肛端上行神经通路。所有标本均录得自发繁殖收缩（0.1 ~ 1.5周期/分钟）。大多数宫缩在所有录制地点同步发生。在五个标本中，有节奏的顺行收缩传播了整个准备长度。电刺激诱发局部收缩（潜伏期5.5±2.4s），幅度大于自发性收缩（电刺激；29.3±26.9 vs 12.1±14.8mmHg； P=0.02）。同步或逆行传播运动模式是EFS的主要特征；71%跨越了整个准备时间。六甲溴铵和利多卡因适度且仅暂时抑制自发性收缩，而TTX增加收缩活动的频率，同时抑制efs诱发的收缩。我们的研究表明，在器官浴中记录的扩散性收缩具有肌源性起源，可由神经输入调节。一旦在局部部位被激活，收缩就不需要粪便的推进来维持长距离的传播。
Colonic high-resolution manometry (HRM) has been used to reveal discrete, propagating colonic motor patterns. To help determine mechanisms underlying these patterns, we used HRM to record contractile activity in human distal colon ex vivo. Surgically excised segments of descending (n = 30) or sigmoid colon (n = 4) were immersed in oxygenated Krebs solution at 36oC (n = 34; 16 Female; 67.6±12.4years; length 24.7±3.5cm;). Contractility was recorded by HRM catheters. After 30 minutes of baseline recording, 0.3mM lidocaine and/or 1mM hexamethonium were applied. Ascending neural pathways were activated by electrical field stimulation (EFS; 10Hz, 0.5ms, 50V, 5s duration) applied to the anal end before and after drug application. Spontaneous propagating contractions were recorded in all specimens (0.1–1.5 cycles/minute). Most contractions occurred synchronously across all recording sites. In five specimens, rhythmic antegrade contractions propagated the full length of the preparation. EFS evoked local contractions at the site of stimulation (latency 5.5±2.4s) with greater amplitude than spontaneous contractions (EFS; 29.3±26.9 vs 12.1±14.8mmHg; P=0.02). Synchronous or retrograde propagating motor patterns followed EFS; 71% spanned the entire preparation length. Hexamethonium and lidocaine modestly and only temporarily inhibited spontaneous contractions, whereas TTX increased the frequency of contractile activity while inhibiting EFS-evoked contractions. Our study suggests that the propagated contractions recorded in the organ bath have a myogenic origin which can be regulated by neural input. Once activated at a local site, the contractions do not require the propulsion of faecal content to sustain long distance propagation.
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