Pseudomonas quinolone signal affects membrane vesicle production in not only gram-negative but also gram-positive bacteria.
Pseudomonas quinolone signal affects membrane vesicle production in not only gram-negative but also gram-positive bacteria.
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DOI：
10.1264/jsme2.me09182
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发表时间：
2010-06
影响因子：
2.2
通讯作者：
Y. Tashiro;S. Ichikawa;T. Nakajima-Kambe;H. Uchiyama;N. Nomura
中科院分区：
文献类型：
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作者：
Y. Tashiro;S. Ichikawa;T. Nakajima-Kambe;H. Uchiyama;N. Nomura
许多革兰氏阴性菌自然产生膜囊泡（MVs）到细胞外环境。喹诺酮假单胞菌信号（PQS）是铜绿假单胞菌的群体感应信号，是MV产生的正调节因子。在这项研究中，我们研究了它对其他革兰氏阴性和阳性细菌产生MV的影响。将PQS添加到大肠杆菌K12培养物中，可以增加MV产量并增大MV。过量的MgCl(2)抑制了有或没有PQS的大肠杆菌MV的产生，这表明细胞表面的阴离子排斥增加了MV的产生。在大肠杆菌的细胞膜和mv中发现了PQS。在其他革兰氏阴性菌中，包括伯克霍尔德氏菌和假单胞菌，PQS也能增强MV的产生。此外，PQS诱导革兰氏阳性细菌枯草芽孢杆菌168产生MV，该细菌在实验室条件下通常不产生MV。过量MgCl(2)不抑制PQS存在下枯草芽孢杆菌MV的产生，提示其产生机制与革兰氏阴性菌不同。综上所述，这些结果表明PQS在革兰氏阴性菌中促进MV的产生，在革兰氏阳性菌中诱导MV的产生。
Many Gram-negative bacteria naturally produce membrane vesicles (MVs) to the extracellular milieu. The Pseudomonas quinolone signal (PQS), a quorum-sensing signal of Pseudomonas aeruginosa, is a positive regulator of MV production. In this study, we investigated its effects on MV production in other Gram-negative and -positive bacterial species. The addition of PQS to an Escherichia coli K12 culture resulted in increased MV production and enlarged MVs. An excessive amount of MgCl(2) repressed E. coli MV production either with or without PQS, suggesting that an anionic repulsion of cellular surfaces increases MV production. PQS was found in the cellular membrane and MVs in E. coli. The enhancement of MV production by PQS occurred in other Gram-negative bacteria, including Burkholderia and Pseudomonas species. Moreover, PQS induced MV production in a Gram-positive bacterium, Bacillus subtilis 168, which does not normally produce MV under laboratory conditions. An excessive amount of MgCl(2) did not repress B. subtilis MV production in the presence of PQS, suggesting the production mechanism to be different from that in Gram-negative bacteria. Together, these results indicated that PQS enhances MV production in Gram-negative bacteria and induces it in Gram-positive bacteria.