NUCLEOTIDE-SEQUENCE OF THE ONCOGENE ENCODING THE P21 TRANSFORMING PROTEIN OF KIRSTEN MURINE SARCOMA-VIRUS
NUCLEOTIDE-SEQUENCE OF THE ONCOGENE ENCODING THE P21 TRANSFORMING PROTEIN OF KIRSTEN MURINE SARCOMA-VIRUS
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DOI：
10.1126/science.6287573
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发表时间：
1982-01-01
期刊：
影响因子：
56.9
通讯作者：
OHTSUBO, E
中科院分区：
文献类型：
--
作者：
TSUCHIDA, N;RYDER, T;OHTSUBO, E
柯尔斯滕鼠肉瘤病毒(Ki-MuSV)的转化蛋白是一种病毒编码的21kD蛋白，称为p21kis。通过分析分子克隆的Ki-MuSV DNA的一系列明确的缺失突变体诱导小鼠细胞局灶性转化的能力，将编码p21kis的序列基因定位于病毒基因组5‘端附近的1.3kb片段。对该区域的一部分进行核苷酸测序，确定了编码p21kis蛋白的567个核苷酸的开放阅读框。
The transforming protein of Kirsten murine sarcoma virus (Ki-MuSV) is a virally encoded 21-kilodalton protein called p21 kis. The sequences encoding p21 kis were genetically localized to a 1.3-kilobase segment near the 5'' end of the viral genome by assaying the capacity of a series of defined deletion mutants of molecularly cloned Ki-MuSV DNA to induce focal transformation of mouse cells. Nucleotide sequencing of a portion of this region has led to the identification of an open reading frame of 567 nucleotides coding for p21 kis protein.