Isolation and characterization of high-temperature-induced Dauer formation mutants in Caenorhabditis elegans.
Isolation and characterization of high-temperature-induced Dauer formation mutants in Caenorhabditis elegans.
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发表时间：
2003-09
期刊：
影响因子：
3.3
通讯作者：
M. Ailion;James H. Thomas
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文献类型：
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作者：
M. Ailion;James H. Thomas
秀丽隐杆线虫的daer形成受至少三种信号通路调控，包括胰岛素受体信号通路。这些途径是由在25度下组成Daf-c的突变体定义的。在25度下对Daf-c突变体的筛选可能已经饱和，但未能识别出调节水形成的所有成分。在这里，我们在27度下筛选Daf-c突变体，这是一种更强烈的诱导条件。发现的突变包括三个已知基因的新型等位基因和至少七个新基因的等位基因，即hid-1-hid-7。许多基因似乎在胰岛素分支中起作用，包括pdk-1， akt-1， aex-6和hid-1。我们还从分子上鉴定了hid-1，并表明它编码了一种新的高度保守的跨膜蛋白，这种蛋白被认为在神经元中表达。
Dauer formation in Caenorhabditis elegans is regulated by at least three signaling pathways, including an insulin receptor-signaling pathway. These pathways were defined by mutants that form dauers constitutively (Daf-c) at 25 degrees. Screens for Daf-c mutants at 25 degrees have probably been saturated, but failed to identify all the components involved in regulating dauer formation. Here we screen for Daf-c mutants at 27 degrees, a more strongly dauer-inducing condition. Mutations identified include novel classes of alleles for three known genes and alleles defining at least seven new genes, hid-1-hid-7. Many of the genes appear to act in the insulin branch of the dauer pathway, including pdk-1, akt-1, aex-6, and hid-1. We also molecularly identify hid-1 and show that it encodes a novel highly conserved putative transmembrane protein expressed in neurons.