A CRITICAL-EVALUATION OF DIRECT ELECTRICAL PROTEIN-DETECTION METHODS
A CRITICAL-EVALUATION OF DIRECT ELECTRICAL PROTEIN-DETECTION METHODS
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DOI：
10.1016/0956-5663(91)85009-l
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发表时间：
1991-01-01
影响因子：
12.6
通讯作者：
BERGVELD, P
中科院分区：
文献类型：
--
作者：
BERGVELD, P
在过去的十年中，许多与蛋白质相关的电现象被研究并应用于各种测量系统，从简单的金属电极吸附蛋白质到复杂的脂质双层系统。许多调查涉及对免疫反应的监测。所观察到的现象的基本潜在电效应是蛋白质调制的介电常数、电导率、电势、离子渗透率和离子迁移率。本文重点介绍了电阻抗测量和场效应管器件的阻抗和电位测量。Donnan理论被处理并应用于静态免疫场效应晶体管操作，解释了已报道的相对较小的影响。最后，描述了一种替代方法，其中免疫场效应管以动态方式应用，以规避静态测量的缺点。
During the last decennia many protein-related electrical phenomena have been studied and applied in a variety of measuring systems, from simple metal electrodes with adsorbed proteins to sophisticated systems with lipid bilayers. Many of the investigations concern the monitoring of immuno reactions. The basic underlying electrical effects of the observed phenomena are the protein modulated dielectric constant, conductivity, electrical potential, ion permeability and ion mobility. In this paper special attention is paid to the capacitive measurements with EIS systems as well as impedance and potential measurements with FET devices. The Donnan theory is treated and applied to the static ImmunoFET operation, explaining the relatively small effects which have been reported. Finally, an alternative approach is described in which the ImmunoFET is applied in a dynamic way, to circumvent the drawbacks of the static measurements.