The mechanism of the hemolytic activity of polyene antibiotics.
The mechanism of the hemolytic activity of polyene antibiotics.
复制标题
多烯类抗生素的溶血活性机制。
DOI：
--
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发表时间：
2002
影响因子：
8.3
通讯作者：
J. Bielawski
中科院分区：
文献类型：
--
作者：
A. Knopik;J. Bielawski
本文研究了非律平、白曲霉素B和制霉菌素诱导人红细胞溶血的动力学。菲律宾引起的溶血是损伤型的。Ca ~（2+）、Ba ~（2+）对该过程有部分抑制作用，而Mg ~（2+）、Na ~+、SO ~（4 2-）对该过程无抑制作用。菲律平的溶血活性可通过在红细胞膜内以大穿孔形式形成大聚集体来解释，小分子量物质以及包括血红蛋白在内的大分子可透过。在等渗KCl溶液中，低浓度的曲霉素B和制霉菌素在膜内形成较小的聚集体。结果，膜对KCl的渗透性增加，并发生溶血。然而，由两种多烯诱导的溶血的动力学是复杂的。该过程具有渗透型特征和损伤型特征。有人建议，Aestericin B和制霉菌素可能同时形成一些运输系统，不同的分子组织和溶血活性。它们对红细胞膜通透性的参与可以通过膜组织和孵育介质的化学成分的微小变化来改变。在二价阳离子氯化物的等渗溶液中，并且在较高的抗生素浓度下，出现允许二价阳离子渗透的额外聚集体。这些结构不允许SO 4（2-）渗透。
The kinetics of the filipin-, amphotericin B- and nystatin-induced hemolysis of human erythrocytes were investigated. Filipin-induced hemolysis is of the damage type. It is an all-or-none process, partly inhibited by Ca2+ or Ba2+ but not by Mg2+, Na+ or SO42-. The hemolytic activity of filipin is explained by the formation of large aggregates within the erythrocyte membrane in the form of large perforations, permeable to substances of low molecular weight as well as to macromolecules, including hemoglobin. In isotonic KCl solution, both amphotericin B and nystatin, at low concentrations, form smaller aggregates within the membranes. As a result, the permeability of the membranes to KCl increases and hemolysis occurs. However, the kinetics of the hemolysis induced by the two polyenes is complex. The process shows some features of the permeability type and some of the damage type. It is suggested that amphotericin B and nystatin may simultaneously form a number of transport systems, differing in their molecular organisation and hemolytic activity. Their participation in erythrocyte membrane permeability can be modified by small changes in membrane organisation and the chemical composition of the incubation medium. In isotonic solutions of divalent cation chlorides, and at higher antibiotic concentration, additional aggregates, allowing divalent cations to permeate, appear. These structures do not permit SO4(2-) to permeate.
使用膜片钳技术表征两性霉素 B 对 MDCK 细胞离子通道的影响。
DOI：
10.1016/s0005-2736(97)00055-2
发表时间：
1997
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Hsu,S;Burnette,RR
通讯作者：
Burnette,RR