Monitoring of cell-free viral DNA in primary Epstein-Barr virus infection
Monitoring of cell-free viral DNA in primary Epstein-Barr virus infection
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原发性 Epstein-Barr 病毒感染中游离病毒 DNA 的监测
DOI：
10.1007/s004300050006
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发表时间：
2000
影响因子：
5.4
通讯作者：
T. Morishima
中科院分区：
文献类型：
--
作者：
H. Kimura;K. Nishikawa;Y. Hoshino;A. Sofue;Y. Nishiyama;T. Morishima
摘要儿童原发性EB病毒感染通常无症状，但有时会引起传染性单核细胞增多症。极偶尔，IM发展成危及生命的EBV相关噬血细胞综合征（EBV-AHS）。我们研究了测量这些原发性EBV感染患者血清中游离病毒DNA的重要性和实用性。使用实时定量聚合酶链反应，无细胞EBV-DNA定量在血清中的9名儿童IM和3与EBV-AHS。在IM的急性期，在95%的血清中检测到平均102.4拷贝/ml的EBV-DNA。EBV负荷逐渐下降，并在1个月内消失。EBV-AHS患者血清中的病毒载量极高（105.5-107.4拷贝/ml）。这些患者的病毒载量持续时间较长，尽管它与症状的改善平行下降。这些结果表明，无细胞EBV-DNA在原发性EBV感染的患者中经常被检测到，因此，可以用作EBV感染的标志物。测定游离EBV-DNA有助于监测原发性EBV感染，尤其是EBV-AHS。
Abstract Primary Epstein-Barr virus (EBV) infection in childhood is usually asymptomatic, but sometimes causes infectious mononucleosis (IM). Very occasionally, IM develops into a life-threatening EBV-associated hemophagocytic syndrome (EBV-AHS). We studied the importance and usefulness of measuring cell-free viral DNA in the serum of patients with these primary EBV infections. Using real-time quantitative polymerase chain reaction, cell-free EBV-DNA was quantified in the serum of nine children with IM and three with EBV-AHS. In the acute phase of IM, an average of 102.4 copies/ml of EBV-DNA was detected in 95% of sera. The EBV load gradually decreased and disappeared within 1 month. Patients with EBV-AHS had an extremely high viral load in their sera (105.5–107.4 copies/ml). The viral load persisted longer in these patients, although it decreased in parallel with the improvement of symptoms. These results indicate that cell-free EBV-DNA was frequently detected in patients with primary EBV infection and could, therefore, be used as a marker for EBV infection. Measuring the cell-free EBV-DNA is useful for monitoring the primary EBV infection, especially in EBV-AHS.