Effects of Environmental Stress on Stability of Tandem Repeats in Escherichia coli O157:H7
Effects of Environmental Stress on Stability of Tandem Repeats in Escherichia coli O157:H7
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DOI：
10.1128/aem.02373-09
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发表时间：
2010-05-01
影响因子：
4.4
通讯作者：
Mandrell, Robert
中科院分区：
文献类型：
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作者：
Cooley, Michael B.;Carychao, Diana;Mandrell, Robert
多位点可变数目串联重复分析（MLVA）用于农业环境中大肠杆菌O157:H7的来源追踪。串联重复序列在有限复制后是稳定的，但在暴露于辐照、高温和饥饿条件下会发生变化。在这些胁迫条件下，pO157质粒经常丢失。根据MLVA测量，环境胁迫可能增加系统发育多样性。
Multilocus variable-number tandem-repeat analysis (MLVA) is used for source tracking Escherichia coli O157:H7 in agricultural environments. Tandem repeats were stable after limited replication but changed after exposure to irradiation, elevated temperatures, and starvation conditions. The pO157 plasmid was frequently lost under these stress conditions. Environmental stresses may increase phylogenetic diversity as measured by MLVA.