Electrotransformation of highly DNA-restrictive Corynebacteria with synthetic DNA
Electrotransformation of highly DNA-restrictive Corynebacteria with synthetic DNA
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DOI：
10.1006/plas.1996.0007
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发表时间：
1996-01-01
期刊：
影响因子：
2.6
通讯作者：
Leblon, G
中科院分区：
文献类型：
--
作者：
Ankri, S;Reyes, O;Leblon, G
高DNA限制性杆状菌可以通过PCR扩增含有pBL1（许多杆状菌共有的质粒）复制起始序列的DNA在体外转化。在所检测的所有菌株中，pcr合成DNA的转化效率等于或提高了从大肠杆菌野生型和dam(-)-dcm（-）菌株中提取的异源DNA的转化效率。用聚合酶链反应制造的DNA获得的转化效率可能足够高，可以允许其普遍应用于基因整合实验。(C) 1996学术出版社，Inc.
Highly DNA-restrictive Corynebacteria can be transformed with DNA made in vitro by PCR amplification of a sequence that contains the replication origin of pBL1, a plasmid common to many Corynebacteria. In all strains examined, the transformation efficiencies of PCR-synthetized DNA equal or improve the performances of heterologous DNA extracted from wild-type and dam(-)-dcm(-) strains of Escherichia coli. The transformation efficiencies obtained with PCR-made DNA may be high enough to permit its general application to experiments of gene integration. (C) 1996 Academic Press, Inc.