Cholesterol doping induced enhanced stability of bicelles
Cholesterol doping induced enhanced stability of bicelles
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DOI：
10.1021/la0345183
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发表时间：
2003-11-11
期刊：
影响因子：
3.9
通讯作者：
Tachibana, K
中科院分区：
文献类型：
--
作者：
Sasaki, H;Fukuzawa, S;Tachibana, K
为了评估作为模型膜系统的bicelles，我们研究了由膜裂解肽蜂毒肽诱导的bicelles的形态变化。P-31 NMR和动态光散射实验表明，蜂毒肽不可逆地破坏了双胞的盘状结构，并且破坏的双胞在凝胶-液晶转变温度（Tm = 24 ℃）以上形成巨大的球形组装体。蜂毒肽诱导的破坏的bicelles被抑制的胆固醇的加入，这表明胆固醇有效地提高了稳定性的bicelles膜以相同的方式在囊泡。此外，双胞结合蜂毒素的位置和它在双胞组装中的作用也进行了讨论。
To evaluate bicelles as a model membrane system, we examined the morphological changes of bicelles induced by the membrane-lytic peptide melittin. P-31 NMR and dynamic light scattering experiments showed that melittin irreversibly disrupted the disk-shaped structure of bicelles and that the disrupted bicelles form giant spherical assemblies above the gel-to-liquid-crystalline transition temperature (T-m = 24 degreesC). The melittin-induced disruption of bicelles was suppressed by the addition of cholesterol, suggesting that cholesterol effectively improved the stability of the bicelle membranes in the same manner as in vesicles. Furthermore, the location of bicelle-bound melittin and its role in bicelle assembly are also discussed.