Fast and sensitive quantification of protein SUMOylation using a dual luciferase biosensor
Fast and sensitive quantification of protein SUMOylation using a dual luciferase biosensor
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使用双荧光素酶生物传感器快速、灵敏地定量蛋白质 SUMO 化
DOI：
10.1039/c6an02479c
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发表时间：
2017
期刊：
影响因子：
4.2
通讯作者：
Wang Hanzhong
中科院分区：
文献类型：
--
作者：
Zhang Yuan;Ke Xianliang;Liu Yan;Zheng Zhenhua;Meng Jin;Wang Hanzhong
在此，开发了一种方便的策略来定量蛋白质SUMO化。目的蛋白和与之偶联的SUMO蛋白的量可以通过基于内切酶的生物发光检测来定量，并且相对生物发光用于评估目的蛋白的SUMO化程度。
Herein a convenient strategy was developed to quantify protein SUMOylation. The amount of target proteins and SUMO proteins conjugated to them could be quantified through luciferase-based bioluminescence detection and the relative bioluminescence was used to evaluate the SUMOylation degree of the target proteins.