Pan-Filovirus Serum Neutralizing Antibodies in a Subset of Congolese Ebolavirus Infection Survivors
Pan-Filovirus Serum Neutralizing Antibodies in a Subset of Congolese Ebolavirus Infection Survivors
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DOI：
10.1093/infdis/jiy453
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发表时间：
2018-12-15
影响因子：
6.4
通讯作者：
Rimoin, Anne W.
中科院分区：
文献类型：
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作者：
Bramble, Matthew S.;Hoff, Nicole;Rimoin, Anne W.
在2014年刚果民主共和国博恩德卫生区爆发扎伊尔埃博拉病毒(EBOV)一年后，我们试图确定对包括EBOV、Bundibugyo(BDBV)、苏丹(SUDV)和马尔堡(Marv)病毒在内的各种丝状病毒的免疫反应的广度。在对15名幸存者进行评估后，5人对多种埃博拉病毒以及在某些情况下对马尔堡病毒表现出一定程度的反应性。5例存活者均对EBOV糖蛋白(GP)和EBOV VP40呈免疫反应，4例对EBOV核蛋白(NP)呈免疫反应。其中3名幸存者对所测试的3种埃博拉病毒GPs(EBOV、BDBV、SUDV)表现出血清学反应。在空斑减少中和试验中，所有5个样本都显示出使用活病毒中和EBOV的能力。值得注意的是，这些EBOV幸存者中有3人对Marv GP有血浆抗体反应。在假病毒中和试验中，这些幸存者中的一部分的血清抗体也中和了EBOV、BDBV、SUDV和大林病毒以及MARV。总而言之，这些发现表明，一些自然获得的埃博拉病毒感染的幸存者不仅产生了泛埃博拉病毒反应，而且在不太常见的情况下，也出现了泛丝状病毒中和反应。
One year after a Zaire ebolavirus (EBOV) outbreak occurred in the Boende Health Zone of the Democratic Republic of the Congo during 2014, we sought to determine the breadth of immune response against diverse filoviruses including EBOV, Bundibugyo (BDBV), Sudan (SUDV), and Marburg (MARV) viruses. After assessing the 15 survivors, 5 individuals demonstrated some degree of reactivity to multiple ebolavirus species and, in some instances, Marburg virus. All 5 of these survivors had immunoreactivity to EBOV glycoprotein (GP) and EBOV VP40, and 4 had reactivity to EBOV nucleoprotein (NP). Three of these survivors showed serologic responses to the 3 species of ebolavirus GPs tested (EBOV, BDBV, SUDV). All 5 samples also exhibited ability to neutralize EBOV using live virus, in a plaque reduction neutralization test. Remarkably, 3 of these EBOV survivors had plasma antibody responses to MARV GP. In pseudovirus neutralization assays, serum antibodies from a subset of these survivors also neutralized EBOV, BDBV, SUDV, and Tai Forest virus as well as MARV. Collectively, these findings suggest that some survivors of naturally acquired ebolavirus infection mount not only a pan-ebolavirus response, but also in less frequent cases, a pan-filovirus neutralizing response.