In vitro assessment of laser sintered bioactive glass scaffolds with different pore geometries
In vitro assessment of laser sintered bioactive glass scaffolds with different pore geometries
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DOI：
10.1108/rpj-12-2014-0175
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发表时间：
2015-01-01
影响因子：
3.9
通讯作者：
Hilmas, Greg
中科院分区：
文献类型：
--
作者：
Kolan, Krishna C. R.;Thomas, Albin;Hilmas, Greg
目的-本文的目的是利用选择性激光烧结（SLS）工艺制造具有复杂孔形状的支架，并研究孔几何形状的影响。用于骨修复的支架的孔隙几何形状是用于确定骨再生率的最重要的参数之一。设计/方法/途径-具有五种不同架构的支架，具有约50%的孔隙率，与硅酸盐（13-93）和硼酸盐（13- 93 B3）为基础的生物活性玻璃使用SLS工艺制造。建立晚期成骨细胞/早期骨细胞细胞系，用于进行细胞增殖试验的支架。细胞接种的支架进行孵育两个，四个和六天，然后通过MTT测定，以量化的代谢活性cells.Findings -结果表明，细胞增殖显着更多的模仿骨小梁结构的支架相比，传统的晶格结构。SLS制造的支架的表面粗糙度驱动初始的细胞增殖，其次是曲率驱动的细胞proliferation.Originality/值-有很少的研究孔的几何形状对组织生长的影响，现有的报告不提供明确的迹象。我们使用生物活性玻璃支架，而不是使用生物聚合物或钛基支架。从这项研究中获得的结果增加了对孔几何形状对细胞增殖的影响的理解，这是基于实验数据和支架表面曲率的分析。
Purpose - The purpose of this paper is to utilize the selective laser sintering (SLS) process to fabricate scaffolds with complex pore shapes and investigate the effects of pore geometry in vitro. The pore geometry of scaffolds intended for use in bone repair is one of the most important parameters used to determine the rate of bone regeneration.Design/methodology/approach - Scaffolds with five different architectures, having approximately 50 per cent porosity, were fabricated with silicate (13-93) and borate (13-93B3)-based bioactive glasses using the SLS process. An established late-osteoblasts/early-osteocytes cell line was used to perform cell proliferation tests on the scaffolds. The cell-seeded scaffolds were incubated for two, four and six days followed by MTT assay to quantify the metabolically active cells.Findings - The results indicated that the cells proliferate significantly more on the scaffolds which mimic the trabecular bone architecture compared to traditional lattice structures. The surface roughness of the SLS-fabricated scaffolds drives the initial cell proliferation which is followed by curvature-driven cell proliferation.Originality/value - There have been very few studies on the effects of pore geometry on tissue growth and the existing reports do not provide clear indications. Instead of using bio-polymer or titanium-based scaffolds, we use bioactive glass scaffolds. The results obtained from this study add to the understanding of the effect of pore geometry on cell proliferation, which is based on the experimental data and analysis of the scaffolds' surface curvature.