POLYCYCLIC AROMATIC HYDROCARBON-DNA AND PROTEIN ADDUCTS IN COAL-TAR TREATED PATIENTS AND CONTROLS AND THEIR RELATIONSHIP TO GLUTATHIONE-S-TRANSFERASE GENOTYPE
POLYCYCLIC AROMATIC HYDROCARBON-DNA AND PROTEIN ADDUCTS IN COAL-TAR TREATED PATIENTS AND CONTROLS AND THEIR RELATIONSHIP TO GLUTATHIONE-S-TRANSFERASE GENOTYPE
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DOI：
10.1016/0165-1161(95)90001-2
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发表时间：
1995-04-01
期刊：
影响因子：
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通讯作者：
DELEO, VA
中科院分区：
文献类型：
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作者：
SANTELLA, RM;PERERA, FP;DELEO, VA
使用煤焦油治疗的牛皮癣患者作为模型人群来评估一组用于监测苯并[a]芘 (BP) 和相关多环芳烃 (PAH) 暴露的免疫测定。这些测定包括通过竞争性酶联免疫吸附测定 (ELISA) 和荧光终点检测来测量白细胞中的 PAH 二醇环氧化物 - DNA 加合物，通过竞争性 ELISA 和颜色终点检测来测量 PAH - 白蛋白加合物，以及通过非竞争性彩色 ELISA 来识别 BP 二醇环氧化物 - DNA 加合物的抗体的血清水平。与对照组相比（4.90 +/- 8.81/10(8)，p = 0.12），患者的 PAH-DNA 加合物（平均 6.77 +/- 12.05/10(8)）升高。患者（平均 0.61 +/- 0.31 fmol/μg）和对照组（0.63 +/- 0.30 fmol/μg）之间的 PAH-白蛋白加合物没有差异。还测定了谷胱甘肽 S-转移酶 M1 基因型，但未发现该基因的存在与 DNA 或蛋白质加合物水平之间存在任何关系。约 30% 的患者和对照者均具有可测量滴度的识别 BPDE-I-DNA 加合物的抗体。通过 ELISA 测量白细胞 DNA 加合物是检测煤焦油治疗的牛皮癣患者 PAH 暴露的最灵敏方法。
Coal tar treated psoriasis patients were used as a model population to evaluate a panel of immunoassays for monitoring exposure to benzo[a]pyrene (BP) and related polycyclic aromatic hydrocarbons (PAH). The assays included measurement of PAH diol epoxide-DNA adducts in white blood cells by competitive enzyme-linked immunosorbent assay (ELISA) with fluorescence endpoint detection, PAH-albumin adducts by competitive ELISA with color endpoint detection and serum levels of antibodies recognizing BP diol epoxide-DNA adducts by noncompetitive color ELISA. PAH-DNA adducts by ELISA were elevated in patients (mean 6.77 +/- 12.05/10(8)) compared to controls (4.90 +/- 8.81/10(8), p = 0.12). There was no difference in PAH-albumin adducts between patients (mean 0.61 +/- 0.31 fmol/mu g) and controls (0.63 +/- 0.30 fmol/mu g). Glutathione S-transferase M1 genotype was also determined but no relationship was found between presence of the gene and either DNA or protein adduct levels. About 30% of both patients and controls had measurable titer of antibodies recognizing BPDE-I-DNA adducts. Measurement of white blood cell DNA adducts by ELISA was the most sensitive method for detecting PAH exposure in coal tar-treated psoriasis patients.