JASMONIC ACID DISTRIBUTION AND ACTION IN PLANTS - REGULATION DURING DEVELOPMENT AND RESPONSE TO BIOTIC AND ABIOTIC STRESS
JASMONIC ACID DISTRIBUTION AND ACTION IN PLANTS - REGULATION DURING DEVELOPMENT AND RESPONSE TO BIOTIC AND ABIOTIC STRESS
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DOI：
10.1073/pnas.92.10.4114
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发表时间：
1995-05-09
影响因子：
11.1
通讯作者：
MULLET, JE
中科院分区：
文献类型：
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作者：
CREELMAN, RA;MULLET, JE
茉莉酸（JA）是一种存在于高等植物中的天然生长调节剂，具有多种生理功能为了准确测定植物组织中的JA水平，我们合成了含有C-13的JA，用作同位素组成为[225]的内标物：[224]：0.98：0.02，天然物质为[225]：[224]：0.15：0.85，气相色谱分析（火焰离子化检测器和质谱）表明内标物由92%的2-（+/-）-[C-13]JA和8%的2-（+/-）-7-iso-[C-13]JA组成。在大豆植株中，JA水平在幼叶、花和果实中最高（果皮中最高）。在大豆种子和幼苗中，JA水平在最年轻的器官中最高，包括下胚轴钩、胚芽和12-h轴。在脱水导致鲜重减少15%的大豆叶片中，JA水平在2 h内增加约5倍，到4 h下降至约对照水平。在脱落酸积累达到最大值之前存在1-2 h的滞后时间。这些结果将在JA生物合成的多途径以及JA在植物发育和对环境信号的响应中的作用的背景下进行讨论。
Jasmonic acid (JA) is a naturally occurring growth regulator found in higher plants, Several physiological roles have been described for this compound (or a related compound, methyl jasmonate) during plant development and in response to biotic and abiotic stress, To accurately determine JA levels in plant tissue, we have synthesized JA containing C-13 for use as an internal standard with an isotopic composition of [225]:[224] 0.98:0.02 compared with [225]:[224] 0.15:0.85 for natural material, GC analysis (flame ionization detection and MS) indicate that the internal standard is composed of 92% 2-(+/-)-[C-13]JA and 8% 2-(+/-)-7-iso-[C-13]JA. In soybean plants, JA levels were highest in young leaves, flowers, and fruit (highest in the pericarp). In soybean seeds and seedlings, JA levels were highest in the youngest organs including the hypocotyl hook, plumule, and 12-h axis, In soybean leaves that had been dehydrated to cause a 15% decrease in fresh weight, JA levels increased approximate to 5-fold within 2 h and declined to approximately control levels by 4 h, In contrast, a lag time of 1-2 h occurred before abscisic acid accumulation reached a maximum, These results will be discussed in the context of multiple pathways for JA biosynthesis and the role of JA in plant development and responses to environmental signals.