PRODUCTION OF INTERLEUKIN-1 AND TUMOR NECROSIS FACTOR BY HUMAN PERIPHERAL MONOCYTES ACTIVATED BY PERIODONTAL BACTERIA AND EXTRACTED LIPOPOLYSACCHARIDES
PRODUCTION OF INTERLEUKIN-1 AND TUMOR NECROSIS FACTOR BY HUMAN PERIPHERAL MONOCYTES ACTIVATED BY PERIODONTAL BACTERIA AND EXTRACTED LIPOPOLYSACCHARIDES
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DOI：
10.1177/00220345880670081401
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发表时间：
1988-08-01
影响因子：
7.6
通讯作者：
ROTHERMEL, H
中科院分区：
文献类型：
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作者：
LINDEMANN, RA;ECONOMOU, JS;ROTHERMEL, H
对与青少年和成人牙周炎相关的革兰氏阴性杆菌及其各自提取的脂多糖(LPS)激活静止的人外周血单核细胞的能力进行了测试。所有致病的革兰氏阴性细菌和所有被测试的内毒素都能够诱导大量的IL-1和TNF的产生，这是已知的诱导破骨细胞性骨吸收的单一因子。与任何形式的牙周病无关的塞尼氏嗜血杆菌不能激活单核细胞。伴放线放线杆菌Y4中纯化的内毒素可在1~10 ng/m L的阈值浓度范围内诱导IL-1和TNF的释放。为了研究全菌激活单核细胞的机制，我们在与戊二醛固定的细菌的培养中加入了聚合菌素B来结合内毒素。这导致IL-1和肿瘤坏死因子的产生减少。为了比较革兰氏阳性口腔细菌对单核细胞的影响，我们还检测了表皮葡萄球菌和革兰氏阳性两亲性脂多糖，即从金黄色葡萄球菌中提取的脂磷壁酸(LTA)。而整个革兰氏阳性菌对单核细胞无刺激作用，LTA在与内毒素相当的浓度范围内可诱导IL-1和TNF的产生。这些结果表明，单核细胞被游离的内毒素或与革兰氏阴性牙周病原菌结合的内毒素激活，产生单因子，可能参与牙周骨的破坏。
Whole Gram-negative bacteria associated with juvenile and adult periodontitis, and their respective extracted lipopolysaccharides (LPS), were tested for the ability to activate quiescent human peripheral blood monocytes. All pathogenic Gram-negative bacteria and all LPS tested were able to induce the production of significant amounts of IL-1 and TNF, monokines known to induce osteoclastic bone resorption. Haemophilus segnis, which has not been associated with any form of periodontal disease, did not activate monocytes. Purified LPS from Actinobacillus actinomycetemcomitans Y4 was able to elicit IL-1 and TNF release at a threshold concentration of 1-10 ng/mL. To examine the mechanism whereby whole bacteria activated monocytes, we added polymixin B in culture with glutaraldehyde-fixed bacteria to bind LPS. This resulted in the abrogation of IL-1 and TNF production. To compare the effects of Gram-positive oral bacteria on monocytes, we also tested Staphylococcus epidermidis and the Gram-positive amphipathic equivalent of LPS, lipoteichoic acid (LTA) extracted from Staphylococcus aureus bacteria. Whereas whole Gram-positive bacteria had no stimulatory effect on monocytes, LTA induced IL-1 and TNF production at a concentration range equivalent to that of the LPS. These results indicate that monocytes are activated by free LPS or LPS bound to Gram-negative pathogenic periodontal bacteria to produce monokines which may contribute to the destruction of periodontal bone.