Diallyl Trisulfide Inhibits Platelet Aggregation through the Modification of Sulfhydryl Groups
Diallyl Trisulfide Inhibits Platelet Aggregation through the Modification of Sulfhydryl Groups
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二烯丙基三硫化物通过巯基修饰抑制血小板聚集
DOI：
10.1021/acs.jafc.9b05557
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发表时间：
2020
影响因子：
6.1
通讯作者：
Taiichiro Seki
中科院分区：
文献类型：
--
作者：
Takashi Hosono;Asuka Sato;Natsumi Nakaguchi;Yori Ozaki-Masuzawa;Taiichiro Seki
二烯丙基三硫化物（DATS）是大蒜素的次级代谢产物，大蒜素是一种挥发性有机硫调味化合物，由大蒜粉碎产生。这些化合物具有各种药用效果，例如抗血小板活性。在这项研究中，我们首次证明了DATS和二丙基三硫（DPTS），这是一个饱和的类似物DATS抑制血小板聚集的细胞机制。将洗涤的小鼠血小板与这些硫化物一起孵育，并通过光透射聚集测定法评价血小板聚集。使用液相色谱-质谱法测量由DATS、DPTS和谷胱甘肽（GSH）产生的反应产物的量。与DPTS相比，DATS能有效地抑制凝血酶、U46619和胶原诱导的血小板聚集，常用于巯基修饰的N-乙基马来酰亚胺（NEM）也能抑制血小板聚集。DATS与GSH的反应活性高于DPTS。提示DATS通过巯基反应抑制血小板聚集。
Diallyl trisulfide (DATS) is a secondary metabolite of allicin, a volatile organosulfur flavoring compound generated by the crushing of garlic. These compounds have various medicinal effects such as antiplatelet activity. In this study, we demonstrated for the first time the cellular mechanism involved in the inhibition of platelet aggregation by DATS and dipropyl trisulfide (DPTS), which is a saturated analogue of DATS. Washed murine platelets were incubated with these sulfides, and platelet aggregation was evaluated by light transmission aggregometry. The amount of reaction products produced by DATS, DPTS, and glutathione (GSH) was measured using liquid chromatography–mass spectrometry. Compared with DPTS, DATS potently inhibited platelet aggregation induced by thrombin, U46619, and collagen.N-Ethylmaleimide (NEM), which is commonly used to modify sulfhydryl groups, also suppressed platelet aggregation. The reactivity of DATS with GSH was higher than that of DPTS. These data suggested that DATS inhibited platelet aggregation through the reaction of sulfhydryl groups.