Distribution and release of peptide YY in dog measured by specific radioimmunoassay.
Distribution and release of peptide YY in dog measured by specific radioimmunoassay.
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DOI：
10.1016/0016-5085(85)90144-1
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发表时间：
1985-03
期刊：
影响因子：
29.4
通讯作者：
I. Taylor
中科院分区：
文献类型：
--
作者：
I. Taylor
多肽YY(PYY)、神经肽Y和胰多肽(PP)是从脑肠轴提取的第三个结构相关多肽家族。在本研究中，我们验证了一种灵敏和特异的PYY测定方法，并用它来测量PYY在犬胃肠道中的分布。此外，我们还比较了PYY和PP对胃内进餐和胰岛素低血糖的反应。犬回肠和结肠组织中PYY浓度最高，分别为1610±123 ng/g和1607±194 ng/g。餐后循环PP和PYY浓度均显著升高(平均ΔPP=180±43pg/ml；平均ΔPYY=205±61pg/ml)。相反，只有血清PP浓度(平均ΔPP=294±36pg/ml)在胰岛素低血糖时显著升高(p<；0.01)，表明PP和PYY细胞对迷走神经刺激的敏感性不同。目前的PYY释放对生理刺激的反应满足了荷尔蒙状态的先决条件之一。
Peptide YY (PYY), neuropeptide Y, and pancreatic polypeptide (PP) are the third family of structurally related peptides to be extracted from the brain-gut axis. In the present study we have validated a sensitive and specific assay for PYY and used it to measure the distribution of PYY in the canine gastrointestinal tract. In addition we have compared the PYY and PP responses to both an intragastric meal and to insulin hypoglycemia. The highest concentration of PYY was present in the canine ileum (1610 ± 123 ng/g) and colon (1607 ± 194 ng/ g). Significant (p < 0.05) increases in both circulating PP and PYY concentrations were observed in response to the meal (mean ΔPP = 180 ± 43 pg/ml; mean ΔPYY = 205 ± 61 pg/ml). In contrast, only serum PP concentrations (mean ΔPP = 294 ± 36 pg/ ml) increased significantly (p < 0.01) in response to insulin hypoglycemia, demonstrating differences in the sensitivity of the PP and PYY cells to vagal stimulation. The present demonstration of PYY release in response to a physiological stimulus fulfills one of the prerequisites for hormonal status.