Transcription expression and clinical significance of vascular endothelial growth factor mRNA and endostatin mRNA in pleural effusions of patients with lung cancer
Transcription expression and clinical significance of vascular endothelial growth factor mRNA and endostatin mRNA in pleural effusions of patients with lung cancer
复制标题
肺癌患者胸腔积液中血管内皮生长因子mRNA和内皮抑素mRNA的转录表达及临床意义
DOI：
10.1002/dc.21546
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发表时间：
2012-04-01
影响因子：
1.3
通讯作者：
Wu, Guang-Ping
中科院分区：
文献类型：
--
作者：
Chen, Ying;Liang, Bin;Wu, Guang-Ping
本研究的目的是评估肺癌患者胸腔积液中血管内皮生长因子（VEGF）mRNA和内皮抑素mRNA的单独和联合诊断效用。应用逆转录聚合酶链反应（RT-PCR）检测92例肺癌和36例肺良性疾病患者胸腔积液中VEGF和Endostatin的转录水平。VEGF和Endostatin mRNA在恶性、AC和SCC积液中的表达均显著高于良性积液（P < 0.01）。在各亚组中，VEGFmRNA在癌性渗出液和AC渗出液中均明显高于EndostatinmRNA（P < 0.01），而VEGFmRNA和EndostatinmRNA在AC组和SCC组间差异无统计学意义（P > 0.05）。单项检测中，VEGFmRNA的敏感性最高（82.6%），准确性最高（84.3%），而内皮抑素mRNA的特异性最高（100%）。当VEGF mRNA和内皮抑素mRNA的组合一起评估时，它们给出了高诊断性能：灵敏度分别为95.7%和93.8%的准确性。VEGF mRNA和Endostatin mRNA的检测可用于鉴别癌细胞和反应性间皮细胞，对胸膜微转移的诊断有一定价值。细胞病理学诊断。2010. (C)2010 Wiley-Liss，Inc.
The aim of this study was to evaluate the individual and combined diagnostic utility of vascular endothelial growth factor (VEGF) mRNA and endostatin mRNA in pleural effusions of patients with lung cancer. Transcription levels of VEGF and endostatin were detected by reverse transcription polymerase chain reaction (RT-PCR) in pleural effusions of patients with lung cancer (92 cases) and with lung benign disease (36 cases). Both VEGF mRNA and endostatin mRNA was significantly higher in malignant, AC, and SCC effusions than in benign effusions (P < 0.01). In the subgrouping, VEGF mRNA was obviously higher than endostatin mRNA in malignant and AC effusions (P < 0.01), whereas VEGF mRNA and endostatin mRNA did not differ between AC group and SCC group (P > 0.05). In single, VEGF mRNA had the highest sensitivity (82.6%) and accuracy (84.3%), whereas endostatin mRNA had the highest specificity (100%). When combinations of VEGF mRNA and endostatin mRNA were evaluated together, they gave a high-diagnostic performance: sensitivity of 95.7% and accuracy of 93.8%, respectively. The detection of VEGF mRNA and endostatin mRNA appears to be suitable for distinguishing carcinoma cells from reactive mesothelial cells in pleural effusions, they could be useful to diagnose the pleural micrometastasis. Diagn. Cytopathol. 2010. (C) 2010 Wiley-Liss, Inc.