Susceptibility of aged mice to Staphylococcus aureus keratitis
Susceptibility of aged mice to Staphylococcus aureus keratitis
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DOI：
10.1080/02713680490516783
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发表时间：
2004-10-01
影响因子：
2
通讯作者：
O'Callaghan, RJ
中科院分区：
文献类型：
--
作者：
Girgis, DO;Sloop, GD;O'Callaghan, RJ
目的.确定年龄对小鼠葡萄球菌性角膜炎发病程度的影响。将幼龄和老龄小鼠（BALB/c、A/J和C57 BL/6）的角膜划痕并局部接种S.金黄色。分别行裂隙灯检查（SLE）和组织病理学检查，并测定细菌菌落形成单位和髓过氧化物酶活性。感染后第1天和第3天，老年小鼠感染眼的SLE评分显著高于感染的年轻小鼠。在所有老年小鼠中观察到的组织学变化比年轻小鼠更严重。年轻的BALB/c和A/J小鼠在PI第3天表现出最小的角膜炎体征，而两种品系的老年小鼠在第3天表现出严重的角膜炎。年轻的C57 BL/6小鼠没有表现出角膜炎的临床体征，而老年C57 BL/6小鼠表现出中度角膜炎。老龄小鼠S.与年轻小鼠相比，金黄色角膜炎表现出增加的病理学。
Purpose. To determine the effect of age on the extent of pathogenesis of Staphylococcus keratitis in the Mouse.Methods. Corneas of young and aged mice (BALB/c, A/J, and C57BL/6) were scarified and topically inoculated with S. aureus. Slit lamp examination (SLE) and histopathology were performed, and bacterial colony forming units and myeloperoxidase activity were determined.Results. SLE scores of infected eyes of aged mice were significantly higher at days 1 and 3 postinfection (PI) as compared to infected young mice. Histopathological changes observed in all aged mice were more severe than those in young mice. Young BALB/c and A/J mice demonstrated minimal signs of keratitis by day 3 PI, whereas aged mice of both strains demonstrated severe keratitis by day 3. Young C57BL/6 mice showed no clinical signs of keratitis, whereas aged C57BL/6 mice demonstrated moderate keratitis.Conclusions. Aged mice with S. aureus keratitis demonstrated increased pathology as compared to young mice.