The comparison of multilineage differentiation of bone marrow and adipose-derived mesenchymal stem cells.
The comparison of multilineage differentiation of bone marrow and adipose-derived mesenchymal stem cells.
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DOI：
10.7754/clin.lab.2012.120312
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发表时间：
2012
影响因子：
0.7
通讯作者：
Xi-shan Zhu;Jing Du;Gang Liu
中科院分区：
文献类型：
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作者：
Xi-shan Zhu;Jing Du;Gang Liu
背景比较脂肪间充质干细胞和骨髓间充质干细胞在多向分化能力中的作用，为干细胞移植提供理论依据。方法分离骨髓间充质干细胞和脂肪间充质干细胞，比较其表型、细胞倍增时间、因子分泌和多向分化能力。结果骨髓间充质干细胞和骨髓间充质干细胞在细胞表型上相似，仅在CD106的表达上存在差异。AMSCs的增殖速度较BMSCs快(倍增时间分别为28h和39h)，抑制T细胞增殖和活化的能力减弱。此外，这两种来源的细胞都能够分化为骨、脂肪和软骨，这证明了它们的干细胞特性。结论细胞来源和丰度是干细胞应用的决定性因素，脂肪组织与AMSCs和BMSCs相同的前提下，是更有前途的干细胞来源。
BACKGROUND To compare the roles of adipose and bone marrow derived mesenchymal stem cells (AMSCs and BMSCs) in multiple differentiation capacity to provide a theoretical basis for stem cell transplantation. METHODS We isolated bone marrow and adipose derived mesenchymal stem cells and compared their phenotype, cell doubling time, the secretion of factors, and the ability of multi-differentiation. RESULTS BMSCs and AMSCs were similar in cell phenotype and the differences existed only between the expression of CD106. The proliferation rate of AMSCs was faster than of BMSCs (doubling time 28h vs. 39h) and the capacity to suppress T cell proliferation and activation was weakened in AMSCs. In addition, both sources of cells were able to differentiate into bone, fat, and cartilage which proved their stem cell properties. CONCLUSIONS Cell origin and abundance were decisive factors in stem cell applications and with the same premise as for AMSCs and BMSCs, adipose tissue is a more promising source of stem cells.