Surface modification of cotton-wool-like bone void fillers consisting of biodegradable polymer-based composite fibers containing calcium-salt particles
Surface modification of cotton-wool-like bone void fillers consisting of biodegradable polymer-based composite fibers containing calcium-salt particles
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DOI：
10.1016/j.rinma.2021.100236
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发表时间：
2021-10
影响因子：
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通讯作者：
Masao Watabe;T. Matsubara;A. Obata;Yasutoshi Nishikawa;T. Kasuga
中科院分区：
文献类型：
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作者：
Masao Watabe;T. Matsubara;A. Obata;Yasutoshi Nishikawa;T. Kasuga
棉絮状骨空隙填充材料是由可生物降解的聚合物（聚（乳酸-共-乙醇酸）; PLGA）与钙盐颗粒（例如碳酸钙和β-型磷酸三钙）组成的缠结纤维的聚集体，其是有利的，因为它可以在手术期间植入缺损区域之前容易地与自体血液和/或骨髓抽吸物（BMA）混合。复合纤维是疏水的，因为表面周围的PLGA相，所以纤维表面的亲水性应该得到改善，以允许血液或BMA与这种棉絮状材料快速混合。在这项工作中，开发了一种在纤维复合材料表面快速形成水溶性薄层的简单方法，以赋予亲水性并提高成骨细胞样细胞的代谢活性。将复合纤维与碳酸钙（球霰石）颗粒机械混合以使它们摩擦带电。将颗粒致密地附着到纤维表面上，然后在60 °C下加热10分钟以使用粘性流动的PLGA将它们固定到表面。将所得纤维在37 °C下浸入0.2mMNa2HPO4水溶液中5分钟。经此处理后，不仅在固定的球文石颗粒上，而且在纤维表面也形成了一层厚度<101 μm的叶状和不规则形状的非晶相。所得的棉絮状材料显示出高亲水性。该新形成的层主要由无定形硅磷酸钠钙相组成。将所得层通过浸入Tris-HCl缓冲溶液中而逐渐溶解。在亲水处理后的棉絮样材料上使用小鼠来源的成骨细胞样细胞MC 3 T3-E1的细胞培养试验显示出显著更高的代谢活性。
A cotton-wool-like bone-void-filling material, an aggregate of tangled fibers consisting of biodegradable polymer (poly(lactic-co-glycolic acid); PLGA) with calcium-salt particles, such as calcium carbonate and β-type tricalcium phosphate, is advantageous because it can be easily mixed with autologous blood and/or bone marrow aspirate (BMA), just before implantation into the defect area during surgery. The composite fibers are hydrophobic because of the PLGA phase around the surface, so hydrophilicity of the fiber surface should be improved to allow blood or BMA to rapidly mix with this cotton-wool-like material. In this work, a simple method for rapidly forming a thin water-soluble layer on the surface of the fibrous composite material was developed to impart hydrophilicity and improve the metabolic activity of osteoblast-like cells. The composite fibers were mechanically mixed with calcium carbonate (vaterite) particles to charge them triboelectrically. The particles were attached densely onto the fiber surface and then heated for 10 min at 60 °C to fix them to the surface using viscous-flowing PLGA. The resulting fibers were immersed in a 0.2 mMNa2HPO4aqueous solution for 5 min at 37 °C. After this treatment, leaf-like and irregularly shaped amorphous phases formed not only on the fixed vaterite particles but also on the fiber surface, as a layer with <∼1μm in thickness. The resulting cotton-wool-like material showed high hydrophilicity. This newly formed layer consisted predominantly of an amorphous sodium calcium silicophosphate phase. The resulting layer was gradually dissolved via immersion in a Tris-HCl buffer solution. A cell culture test using mouse-derived osteoblast-like cells MC3T3-E1 on a cotton-wool-like material after hydrophilic treatment showed significantly higher metabolic activity.