Directional cell movement during early development of the teleost Blennius pholis: I. Formation of epithelial cell clusters and their pattern and mechanism of movement.
Directional cell movement during early development of the teleost Blennius pholis: I. Formation of epithelial cell clusters and their pattern and mechanism of movement.
复制标题
硬骨鱼Blennius pholis早期发育过程中的定向细胞运动：I.上皮细胞簇的形成及其运动模式和机制。
DOI：
10.1002/jez.1402450206
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Trinkaus,JP
中科院分区：
文献类型：
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作者：
Trinkaus,JP
硬骨鱼Blennius pholis的胚胎为观察体内细胞簇的形成和运动提供了特殊的材料，因为这些细胞簇被黑素体包裹并在透明的包膜层下迁移。这些集群产生于两个色素细胞团（PCM）早熟出现在胚胎轴两侧的3/5外包，在体节1和2的未来水平。随着发育的进行，每个PCM在其侧缘扩大并扩散以形成上皮片。随着扩张的继续，片层碎裂，形成在卵黄囊中向远侧方向移动的小细胞簇。伸展PCM的高度活动的侧缘细胞，以及每个移动簇的边缘细胞，总是突出高度扁平的板状伪足，其终止于长，细，通常分支的丝状伪足。当细胞团离开PCM时，它们形成长的、拉紧的收缩纤维。PCM的侧边缘和簇运动的初始阶段的扩展速率高于（1.0 μm/min或更大）簇运动的后期速率，显然是因为在该阶段，运动活动仅限于每个的远端边界。在16°-18 ° C下，这种定向迁移在24 h内停止，此时最远的簇已经到达卵黄囊的腹侧区域。到那时，所有的星团都或多或少地均匀分布，显然是由于所有星团的运动几乎在同一时间停止。一旦运动停止，集群保持不动2-4天，这取决于温度。
Embryos of the teleostBlennius pholisprovide exceptional material for observation of the formation and movement of cell clusters in vivo because the clusters are packed with melano‐somes and migrate beneath the transparent enveloping layer. These clusters arise from two pigmented cell masses (PCM) which appear precociously on either side of the embryonic axis at 3/5 epiboly, at the future level of somites 1 and 2. As development proceeds, each PCM enlarges and spreads on its lateral margins to form an epithelial sheet. As spreading continues, the sheet fragments, forming small cell clusters that move in a distad direction in the yolk sac. The highly motile lateral marginal cells of the spreading PCM, as well as those of the marginal cells of each moving cluster, invariably protrude highly flattened lamellipodia, which terminate in long, fine, often branched filopodia. As cell clusters leave the PCM, they form long, taut retraction fibers. The rate of spreading of both the lateral edge of the PCM and the initial phase of cluster movement, is higher (1.0 μm/min or greater) than the later rate of cluster movement, apparently because at this phase, motile activity is confined to the distal borders of each. This directional migration ceases in 24 h at 16°–18°C, when the farthest clusters have reached the ventral region of the yolk sac. By then, all clusters are spaced more or less evenly, apparently due to cessation of all cluster movement at about the same time. Once movement ceases, the clusters remain immobile for 2–4 days, depending on the temperature.