Early retinal damage in experimental diabetes: Electroretinographical and morphological observations
Early retinal damage in experimental diabetes: Electroretinographical and morphological observations
复制标题
DOI：
10.1006/exer.2002.1170
复制
发表时间：
2002-05-01
影响因子：
3.4
通讯作者：
Perlman, I
中科院分区：
文献类型：
--
作者：
Li, Q;Zemel, E;Perlman, I
越来越多的证据表明，视网膜功能受损先于血管并发症的最早迹象。本研究的目的是在糖尿病大鼠模型中从功能和形态学上跟踪视网膜病变的发展。采用链脲佐菌素（STZ）静脉注射诱导大鼠糖尿病模型。在类似条件下饲养的大鼠作为对照。记录视网膜电图（ERG）以评估视网膜功能。以Muller细胞中胶质细胞酸性蛋白（GFAP）的表达作为视网膜损伤的细胞标志物。早在糖尿病发作后2周，与对照大鼠记录的反应相比，糖尿病大鼠的ERG反应的幅度降低。b波比a波受影响更大。在糖尿病的前5周，糖尿病视网膜中的GFAP表达与对照视网膜中的GFAP表达没有差异。GFAP仅在玻璃体视网膜边缘的星形胶质细胞中表达。糖尿病6-7周后，在视网膜的Muller细胞的终足中也检测到GFAP的表达。随着糖尿病的进展，GFAP的表达遍及整个Muller细胞的长度。在对照组老鼠的视网膜上。GFAP表达仅限于星形胶质细胞，即使在40周的随访中也未在Muller细胞中检测到。观察结果表明，视网膜细胞的功能完整性已经在大鼠实验性糖尿病发作后的短时间间隔内受到损害。(C)2002 Elsevier Science Ltd.
A growing body of evidence indicates that impairment of retinal function precedes the earliest signs of vascular complications. The aim of this study was to follow the development of retinopathy both functionally and morphologically in a rat model of diabetes mellitus. Diabetes was induced in rats by intravenous injection of streptozotocin (STZ). Age-matched rats raised under similar conditions served as control. The electroretinogram (ERG) was recorded in order to assess retinal function. The expression of glial fibrillary acidic protein (GFAP) in Muller cells was used as a cellular marker for retinal damage. The ERG responses of the diabetic rats were reduced in amplitude compared to the responses recorded from the control rats as early as 2 weeks after onset of diabetes. The b-wave was more affected than the a-wave. GFAP expression in the diabetic retina did not differ from that in the control retina during the first 5 weeks of diabetes. GFAP was demonstrated only in astrocytes in the vitreo-retinal border. After 6-7 weeks of diabetes, GFAP expression in the retinas of the diabetic rats was also detected in the endfeet of the Muller cells. With the progression of diabetes, GFAP expression spreads throughout the entire length of the Muller cells. In the retinas from control rats. GFAP expression was limited to astrocytes and was not detected in Muller cells even at 40 weeks of follow-up. The observations indicate that the functional integrity of retinal cells is compromised already at short time intervals after onset of experimental diabetes in rats. (C) 2002 Elsevier Science Ltd.