Vanadium ions stimulate DNA synthesis in Swiss mouse 3T3 and 3T6 cells.
Vanadium ions stimulate DNA synthesis in Swiss mouse 3T3 and 3T6 cells.
复制标题
钒离子刺激瑞士小鼠 3T3 和 3T6 细胞中的 DNA 合成。
DOI：
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发表时间：
1983
影响因子：
11.1
通讯作者：
J. Smith
中科院分区：
文献类型：
--
作者：
J. Smith
Vanadyl sulfate and sodium orthovanadate in the concentration range between 5 and 50 microM are shown to be mitogenic for quiescent cultures of Swiss mouse 3T3 and 3T6 cells. The compounds caused a striking shift in the dose-response for the effect of serum on [3H]thymidine incorporation and DNA synthesis. In the absence of serum the effect of vanadium was greatly potentiated by insulin. Vanadium ions produced no more than additive increases in [3H]thymidine incorporation when combined with epidermal growth factor, cholera toxin, or phorbol 12-myristate 13-acetate. Both vanadium compounds stimulated ouabain-inhibitable 86Rb+ uptake, indicating that the vanadium ions increase, rather than inhibit, Na+/K+ pump activity in the intact cell. Neither vanadium compound had any effect on cellular cAMP under a variety of different conditions. The mitogenic effect of the vanadium compounds was similar to that of colchicine. Taxol, which stabilizes cytoplasmic microtubules, prevented the stimulation of DNA synthesis by vanadium.
