Demethoxycurcumin Retards Cell Growth and Induces Apoptosis in Human Brain Malignant Glioma GBM 8401 Cells.
Demethoxycurcumin Retards Cell Growth and Induces Apoptosis in Human Brain Malignant Glioma GBM 8401 Cells.
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DOI：
10.1155/2012/396573
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Hsu YC
中科院分区：
文献类型：
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作者：
Huang TY;Hsu CW;Chang WC;Wang MY;Wu JF;Hsu YC
去甲氧基姜黄素（DMC；一种与姜黄素相关的去甲氧基化合物）最近被证明具有抗氧化和抗肿瘤活性。它还对癌症产生有效的化学预防作用。本研究在人脑恶性胶质瘤GBM 8401细胞中研究了DMC的抗增殖（通过MTT法，发现DMC对GBM 8401细胞具有细胞毒活性，IC50值为22.71μM）和诱导凋亡作用。我们研究了线粒体膜电位 (MMP)、DNA 碎片、半胱天冬酶激活和 NF-κB 转录因子活性。通过这些方法，我们的结果表明 DMC 对 GBM 8401 细胞的细胞增殖产生抑制作用并激活细胞凋亡。观察到这两种效应均随着 DMC 治疗剂量的增加而成比例增加，并且 DMC 通过线粒体和 caspase 依赖性途径诱导人脑恶性胶质瘤 GBM 8401 细胞凋亡。
Demethoxycurcumin (DMC; a curcumin-related demethoxy compound) has been recently shown to display antioxidant and antitumor activities. It has also produced a potent chemopreventive action against cancer. In the present study, the antiproliferation (using the MTT assay, DMC was found to have cytotoxic activities against GBM 8401 cell with IC50 values at 22.71 μM) and induced apoptosis effects of DMC have been investigated in human brain malignant glioma GBM 8401 cells. We have studied the mitochondrial membrane potential (MMP), DNA fragmentation, caspase activation, and NF-κB transcriptional factor activity. By these approaches, our results indicated that DMC has produced an inhibition of cell proliferation as well as the activation of apoptosis in GBM 8401 cells. Both effects were observed to increase in proportion with the dosage of DMC treatment, and the apoptosis was induced by DMC in human brain malignant glioma GBM 8401 cells via mitochondria- and caspase-dependent pathways.