Ribose 2′-F labeling:: A simple tool for the characterization of RNA secondary structure equilibria by 19F NMR spectroscopy
Ribose 2′-F labeling:: A simple tool for the characterization of RNA secondary structure equilibria by 19F NMR spectroscopy
复制标题
DOI：
10.1021/ja052844u
复制
发表时间：
2005-08-24
影响因子：
15
通讯作者：
Micura, R
中科院分区：
文献类型：
--
作者：
Kreutz, C;Kählig, H;Micura, R
我们提出了一种简便的19 F核磁共振波谱方法来表征的RNA。该概念基于用单个2 '-脱氧-2'-氟（2 '-F）核苷对RNA进行位点特异性标记。根据2 '-F标记的位置（在双螺旋或单链区域内），观察到显着不同的19 F化学位移，并分配给特定的替代折叠。这已经在一系列的cDNA中得到了例证。该方法是有前途的复杂RNA折叠途径的详细调查。
We present a facile19F NMR spectroscopic approach for the characterization of bistable RNA. The concept is based on the site-specific labeling of RNA with single 2‘-deoxy-2‘-fluoro (2‘-F) nucleosides. Depending on the position of the 2‘-Flabel (either within a double helix or a single-stranded region), significantly different19F chemical shifts were observed and assigned to the particular alternative folds. This has been exemplified for a series of bistable RNAs. The approach is promising for the detailed investigation of complex RNA folding pathways.