Comparison of lethality, reproduction, and behavior as toxicological endpoints in the nematode Caenorhabditis elegans
Comparison of lethality, reproduction, and behavior as toxicological endpoints in the nematode Caenorhabditis elegans
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DOI：
10.1080/009841099157179
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发表时间：
1999-12-10
影响因子：
2.6
通讯作者：
Williams, PL
中科院分区：
文献类型：
--
作者：
Dhawan, R;Dusenbery, DB;Williams, PL
本研究描述了一种新的方法来评估有毒物质暴露后的行为变化，并比较了该方法与环境毒理学中使用的其他常见终点。线虫秀丽隐杆线虫暴露于一系列的乙醇浓度，以确定其对生存，繁殖和行为的影响。通过比较乙醇暴露的LC 50、RC 50（与对照组相比，后代数量减少50%的浓度）和BC 50（与对照组相比，运动减少50%的浓度）值，评价每个终点的敏感性。蠕虫在83 g/L至99 g/L的乙醇浓度范围内显示24 h致死率。在乙醇浓度为8 g/L ~ 71 g/L范围内，暴露3d后，随着乙醇浓度的增加，后代数量逐渐减少。通过使用一种新的计算机跟踪方法来量化行为，该方法可以同时评估数百条线虫，并提供真实的时间的几个行为参数。蠕虫在非常低的乙醇浓度（0.8 g/L和2.4 g/Li）下表现出一些活动过度（增加运动），而在较高的乙醇浓度（14 g/L至40 g/Li）下运动减少。对三个终点之间的敏感性进行了比较。行为和生殖反应被发现是相似的，正如预期的那样，两者都是毒性比致死性更敏感的指标。讨论了计算机跟踪系统的优点和缺点。
This study describes a new approach for assessing behavioral changes following toxicant exposure and compares the method to other common endpoints used in environmental toxicology. The nematode Caenorhabditis elegans was exposed to a range of ethanol concentrations to determine its effect on survival, reproduction and behavior. Each endpoint was evaluated for its sensitivity by comparing LC50, RC50 (concentration at which there is a 50% reduction in number of offspring as compared to controls), and BC50 (concentration at which there is a 50% reduction in movement as compared to controls) values for ethanol exposure. Worms showed 24-h lethality at concentrations of ethanol in the range of 83 g/L to 99 g/L. Reproduction in C elegans was estimated by counting the number of off-spring after 3 d of exposure, which decreased with the increase in ethanol concentration from 8 g/L to 71 g/L. Behavior was quantified by using a new computer tracking method which can simultaneously assess hundreds of nematodes and provides several behavioral parameters in real time. Worms showed some hyperactivity increased movement) at very low ethanol concentrations (0.8 g/L and 2.4 g/Li and a decrease in movement at higher ethanol concentrations 14 g/L to 40 g/Li. A comparison for sensitivity between the three endpoints was performed. Behavior and reproduction responses were found to be similar and, as expected, both are much more sensitive indicators of toxicity than lethality. The advantages and disadvantages of the computer tracking system are discussed.