The Evolutionary History of the sinica-Group of Macaque Monkeys as Revealed by mtDNA Restriction Site Analysis
The Evolutionary History of the sinica-Group of Macaque Monkeys as Revealed by mtDNA Restriction Site Analysis
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DOI：
10.1016/1055-7903(92)90018-c
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发表时间：
1992-09-01
影响因子：
4.1
通讯作者：
Melnick, Don J.
中科院分区：
文献类型：
--
作者：
Hoelzer, Guy A.;Hoelzer, Mary A.;Melnick, Don J.
本文对中国猕猴的线粒体DNA单倍型进行了系统发育关系的研究。辐射木M. thibetana，M assamensis，可能还有M.北极熊。我们做了一些努力来检测。种内变异是通过从物种分布区的不同部分或不同母系中取样个体而产生的。在M。assamensis，从两个亚种（M. assamensisassamensis和M. assamensis pelops）。从血液样品中提取总基因组DNA并用一组16种限制性内切酶切割。总共有97个限制性位点被映射为这些酶的sinica组和M nemestrina，这是作为一个外组。最大简约法和邻接法构建的系统发育树一致性较高。最大简约树的自举分析表明，在80%多数规则共识树内的特定单倍型的关联具有很高的置信度。一个详尽的搜索所有可能的树也支持这个拓扑结构，虽然一个单倍型必须从这个分析中删除，以节省计算机时间。结果也不受加权的字符状态的变化，有利于网站收益超过网站损失。在形态学和等位酶方面，这里产生的线粒体DNA同源性与树木有三个不同之处：M。sinica和M.辐射位于树的两个不同分支; M. assamensis彼此分离;和M. arctoides始终落在sinica组的其余部分之外。我们认为，这些物种的地理历史，沿着线粒体DNA单倍型谱系的排序是我们的观察负责。这些结果支持了最近其他几项研究中的结果，表明来自高度多态祖先的物种形成可能会干扰我们重建分支发生历史模式的尝试。减少这个问题的一种方法是在使用mtDNA重建遗传学时包括种内变异。(C)1992年学术出版社
We estimated the phylogenetic relationships of mitochondrial DNA haplotypes within the sinica-group of macaques, which includes Macaca sinica, M. radiata, M. thibetana, M assamensis, and possibly M. arctoides. Some effort was made to detect. intraspecific variation by sampling individuals from different parts of the species' range or from different matrilines. In the case of M. assamensis, individuals were sampled from both subspecies (M. assamensis assamensis and M. assamensis pelops). Total genomic DNA was extracted from blood samples and cut with a battery of 16 restriction endonucleases. A total of 97 restriction sites were mapped for these enzymes in the sinica-group and M nemestrina, which was used as an outgroup. Phylogenetic trees constructed by both the maximum parsimony method and the neighbor-joining method were highly congruent. A bootstrap analysis of the maximum parsimony tree indicated a high degree of confidence to the association of particular haplotypes within the 80% majority rule consensus tree. An exhaustive search of all possible trees also supported this topology, although one haplotype had to be eliminated from this analysis to save computer time. The results were also unaffected by weighting the character state changes in favor of site gains over site losses. The mtDNA phylogeny produced here differs from trees based on morphology and allozymes in three ways: M. sinica and M. radiation sit in two different branches of the tree; the two subspecies of M. assamensis are separated from one another; and M. arctoides consistently fell outside the rest of the sinica-group. We suggest that the biogeographic histories of these species, along with the sorting of mtDNA haplotype lineages is responsible for our observations. These results support those obtained in several other recent studies in indicating that speciation from a highly polymorphic ancestor can interfere with our attempts to reconstruct the historical pattern of cladogenesis. One way to lessen this problem is to include within-species variation when using mtDNA to reconstruct phylogenies. (C) 1992 Academic press, Inc.