Clinical significance of prostaglandin E synthase expression in gastric cancer tissue
Clinical significance of prostaglandin E synthase expression in gastric cancer tissue
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DOI：
10.1016/j.humpath.2007.04.020
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发表时间：
2007-12-01
期刊：
影响因子：
3.3
通讯作者：
Sakamoto, Choitsu
中科院分区：
文献类型：
--
作者：
Gudis, Katya;Tatsuguchi, Atsushi;Sakamoto, Choitsu
已有研究将微粒前列腺素E合成酶(MPGES)-1与胃癌联系起来。本研究的目的是检测mPGES-1、mPGES-2和胞浆pGES(CPGES)在胃癌组织中的表达，探讨mPGES-1和mPGES-2表达与临床病理因素和环氧合酶-2表达的关系。用Western印迹法检测胃癌细胞系和胃癌患者活检组织中PGES蛋白的表达。应用免疫组织化学方法检测129例胃癌手术切除标本中mPGES-1、mPGES-2和cPGES蛋白的定位。MPGES-1蛋白在胃癌活检组织和有分化或未分化腺癌的癌细胞系中均有表达。在非肿瘤性活检组织中未见mPGES-1表达。所有细胞系和组织标本均表达mPGES-2和cPGES。免疫组织化学分析显示47%的病例癌细胞表达mPGES-1。MPGES-2免疫反应在非肿瘤性腺上皮和癌细胞中均可见；然而，在29%的病例中，癌细胞的免疫反应更为明显。CPGES在非肿瘤性组织和肿瘤性组织中均呈结构性表达，不同病例间无显著差异。MPGES-1和mPGES-2的表达与环氧合酶-2的表达相关。多因素分析显示，mPGES-1、mPGES-2的表达和肿瘤转移分期对胃癌患者的预后有独立意义。然而，在低分化癌患者中，只有肿瘤转移分期和mPGES-2的表达具有独立的预后意义。MPGES-1和mPGES-2与胃癌的临床病理因素相关，可作为判断胃癌预后的有价值的指标。(C)2007 Elsevier Inc.保留所有权利。
Studies have linked microsomal prostaglandin E synthase (mPGES)-1 with gastric cancer. The purpose of this study was to determine mPGES-1, mPGES-2, and cytosolic PGES (cPGES) expression in gastric cancer and to evaluate the correlation between mPGES-1 and mPGES-2 expression and clinicopathological factors and cyclooxygenase-2 expression. PGES protein expression was examined by Western blot in gastric cancer cell lines and in biopsy samples from patients with gastric cancer. mPGES-1, mPGES-2, and cPGES protein localizations were examined immunohistochemically in 129 archival gastric cancer surgical resections. mPGES-1 protein expression was found in gastric cancer biopsies and cancer cell lines with differentiated or undifferentiated adenocarcinoma. There was no mPGES-1 expression in nonneoplastic biopsies. All cell lines and tissue samples expressed mPGES-2 and cPGES. Immunohistochemical analysis showed cancer cells expressed mPGES-1 in 47% of cases. mPGES-2 immunoreactivity was seen both in nonneoplastic glandular epithelium and cancer cells; however, cancer cell immunoreactivity was significantly more pronounced in 29% of cases. cPGES expression was constitutive both in nonneoplastic and neoplastic tissues, with no significant variation among cases. mPGES-1 and mPGES-2 expression correlated with cyclooxygenase-2 expression. mPGES-1 and mPGES-2 expression, and tumor-node-metastasis stage had independent prognostic significance under multivariate analysis in patients with gastric cancer overall and in patients with differentiated cancers. However, only tumor-node-metastasis stage and mPGES-2 expression retained independent prognostic significance in patients with poorly differentiated cancers. mPGES-1 and mPGES-2 correlate with clinicopathological factors and may be valuable prognostic factors in gastric cancer. (c) 2007 Elsevier Inc. All rights reserved.