"Lupinosis"-like lesions in mice caused by ustiloxin, produced by Ustilaginoieda virens: A morphological study
"Lupinosis"-like lesions in mice caused by ustiloxin, produced by Ustilaginoieda virens: A morphological study
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DOI：
10.1002/nt.2620020106
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发表时间：
1994-01-01
期刊：
影响因子：
--
通讯作者：
Sato, Zenji
中科院分区：
文献类型：
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作者：
Nakamura, Ken-Ichi;Izumiyama, Naotaka;Sato, Zenji
用甲醇提取稻穗黑穗病病菌（Ustilaginoidea virens）的水提取物得到一种粗毒素，并腹腔注射给小鼠。单次注射100或200 mg/kg体重的提取物不致死，但引起肝细胞和肾小管细胞急性、偶发坏死，有丝分裂象增加，偶见多核巨细胞。1周后观察前胃糜烂溃烂及胸腺萎缩。反复腹腔注射粗毒素12和25 mg/kg体重可引起更严重的肝、肾坏死，有丝分裂发生延迟。前胃糜烂也有发生。连续注射3或6mg /kg粗提取物或400 μ g/kg乌斯蒂洛素A 10-12天，使用纯化晶体，引起相对轻微但明确的肝脏和肾脏病变，类似于上述情况。肝脏和肾脏的病变与由钩端绒斑虫产生的一种真菌毒素——绒斑素A引起的红斑狼疮非常相似。该有毒物质的分离表明，受污染的水稻穗可能引起牛中毒，尽管尚未发生野外暴发。
A crude toxin, obtained by methanol-extraction of a water extract of false smut balls (INA-KOUJI in Japanese) caused by Ustilaginoidea virens on rice panicles, was injected into mice intraperitoneally. Single injection of 100 or 200 mg/kg body weight of the extract was not lethal but caused acute, occasional necrosis of hepatocytes and renal tubular cells, followed by increased number of mitotic figures with occasional multinuclear giant cells. Erosions and ulceration of the forestomach and atrophy of the thymus were observed a week later. Repeated intraperitoneal injection of the crude toxin at the levels of 12 and 25 mg/kg body weight induced more severe necrosis of the liver and kidneys, with delayed occurrence of mitosis. Forestomach erosion also occurred. Serial injection for 10-12 days of either 3 or 6 mg/kg of the crude extract or 400 mu-g/kg of ustiloxin A, using the purified crystals, caused relatively mild but definite liver and kidney lesions similar to those described above. The lesions in the liver and kidney were quite similar to those observed in lupinosis caused by phomopsin A, a mycotoxin produced by Phomopsis leptostromiformis. Isolation of the toxic substance indicates that the contaminated rice panicles may cause toxicosis of cattle, although no field outbreaks have occurred yet.