The genome of the kinetoplastid parasite, Leishmania major
The genome of the kinetoplastid parasite, Leishmania major
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DOI：
10.1126/science.1112680
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发表时间：
2005-07-15
期刊：
影响因子：
56.9
通讯作者：
Myler, PJ
中科院分区：
文献类型：
--
作者：
Ivens, AC;Peacock, CS;Myler, PJ
利什曼原虫在世界热带和亚热带地区引起一系列人类疾病。我们对大利什曼原虫（Friedlin菌株）32.8百万碱基单倍体基因组的36条染色体进行了测序，预测了911个RNA基因、39个假基因和8272个蛋白质编码基因，其中36%可以归因于假定的功能。这些包括参与宿主-病原体相互作用的基因，如蛋白水解酶，以及复杂表面糖结合物合成的广泛机制。大L.、布鲁氏锥虫和克氏锥虫（Tritryp）基因组中蛋白质编码基因组成长链特异性、多顺反子簇和缺乏一般转录因子的研究表明，尽管锥虫似乎能够进行染色质重塑，但其RNA聚合酶ii定向转录的调节机制与其他真核生物不同。Tritryp基因组中编码了丰富的rna结合蛋白，这与基因表达的转录后活性调控一致。
Leishmania species cause a spectrum of human diseases in tropical and subtropical regions of the world. We have sequenced the 36 chromosomes of the 32.8-megabase haploid genome of Leishmania major (Friedlin strain) and predict 911 RNA genes, 39 pseudogenes, and 8272 protein-coding genes, of which 36% can be ascribed a putative function. These include genes involved in host-pathogen interactions, such as proteolytic enzymes, and extensive machinery for synthesis of complex surface glycoconjugates. The organization of protein-coding genes into long strand-specific, polycistronic clusters and lack of general transcription factors in the L. major, Trypanosoma brucei, and Trypanosoma cruzi (Tritryp) genomes suggest that the mechanisms regulating RNA polymerase II-directed transcription are distinct from those operating in other eukaryotes, although the trypanosomatids appear capable of chromatin remodeling. Abundant RNA-binding proteins are encoded in the Tritryp genomes, consistent with active posttranscriptional regulation of gene expression.