EFFECT OF SUBINHIBITORY CONCENTRATIONS OF ANTIBIOTICS ON EXTRACELLULAR SHIGA-LIKE TOXIN-I
EFFECT OF SUBINHIBITORY CONCENTRATIONS OF ANTIBIOTICS ON EXTRACELLULAR SHIGA-LIKE TOXIN-I
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DOI：
10.1007/bf01704889
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发表时间：
1992-01-01
期刊：
影响因子：
7.5
通讯作者：
CLEARY, TG
中科院分区：
文献类型：
--
作者：
WALTERSPIEL, JN;ASHKENAZI, S;CLEARY, TG
肠出血性大肠杆菌(EHEC)(例如O157：H7)引起的腹泻患者在使用抗菌药物治疗时，患溶血性尿毒症综合征的风险可能更高。有人认为，这可能是由于这类生物释放或产生的Vero或志贺氏样毒素增加，可能是对抗菌剂的应激反应。这项研究的目的是用一种新开发的方法在体外检测这种细胞外毒素的增加，这种方法将EHEC暴露在较高的亚致死浓度下，然后在逐渐较低的浓度下进入恢复期。将5株肠出血性大肠杆菌分别暴露于浓度不断变化的环丙沙星、复方新诺明、头孢克辛和四环素中。将释放的游离志贺样毒素I(SLT-I)的量与未接触抗生素的接种组的释放量进行比较。5株肠出血性大肠埃希菌在暴露于抗菌药物后的产毒量有显著差异(p<；0.001)。同样重要的是抗生素的种类(p<；0.001)，环丙沙星引起的增幅最大，从169%到436%不等，其次是复方新诺明、头孢克辛和四环素。此外，游离毒素的增加与抗生素的浓度有关(p<；0.001)。EHEC菌株产生的抗生素诱导的SLT-I增加与某些类别的抗生素之间的关联可能会影响未来对HUS危险因素的流行病学研究的分析。
Patients with diarrhea due to strains of enterohemorrhagic Escherichia coli (EHEC) (e. g. O157:H7) might be at a higher risk of developing hemolytic uremic syndrome when treated with antimicrobial agents. It has been suggested that this might be due to an increase of release or production of vero or shiga-like toxin from such organisms, possibly as a stress response to antimicrobial agents. The aim of this study was to detect such increases in extracellular toxin in vitro with a newly developed method that exposed EHEC to high sublethal concentrations followed by a recovery phase at progressively lower concentrations. Five strains of EHEC were exposed to continuously changing concentrations of ciprofloxacin, co-trimoxazole, cefixime and tetracycline. The amount of free shiga-like toxin I (SLT-I) released was compared to the amount released from inocula that were not exposed to antibiotics. There were significant differences between the five EHEC strains in the amount of toxin detected after exposure to antimicrobial agents (p < 0.001). Equally important was the type of antibiotic (p < 0.001), with ciprofloxacin inducing the largest increase ranging from 169 to 436%, followed by co-trimoxazole, cefixime and tetracycline. In addition, the increases in free toxin correlated with the concentration of the antibiotics (p < 0.001). The association between antibiotic-induced increases in SLT-I produced by strains of EHEC and certain classes of antibiotics might influence the analysis of future epidemiological studies on risk factors for HUS.