γ-aminobutyric acid accumulation enhances the cell growth of Candida glycerinogenes under hyperosmotic conditions
γ-aminobutyric acid accumulation enhances the cell growth of Candida glycerinogenes under hyperosmotic conditions
复制标题
γ-氨基丁酸积累促进高渗条件下产甘油念珠菌的细胞生长
DOI：
10.2323/jgam.2017.08.002
复制
发表时间：
2018-01-01
影响因子：
1.2
通讯作者：
Zhuge, Bin
中科院分区：
文献类型：
--
作者：
Ji, Hao;Lu, Xinyao;Zhuge, Bin
γ -氨基丁酸（GABA）是一种重要的非蛋白氨基酸，参与植物细胞对各种环境胁迫的反应。本研究的目的是验证一种假设，即GABA的细胞内积累提高了非常规酵母菌产甘油假丝酵母的渗透耐受性。在C. glycerinogenes中，编码gaba特异性渗透酶的UGA4的表达受到高渗胁迫的高度诱导。在高渗条件下，外源GABA增加细胞内GABA积累，促进细胞生长。谷氨酸脱羧酶基因GAD1的过度表达导致高渗条件下细胞内GABA的增加和细胞生长的改善。这些结果表明，无论是通过外源施用还是通过细胞合成，提高产甘油原菌胞内GABA积累，都有助于提高其对高渗胁迫的耐受性。我们证明了GABA积累在非常规酵母抗渗透胁迫中起重要作用。
gamma-aminobutyric acid (GABA) is an important nonprotein amino acid involved in the response to various environmental stresses in plant cells. The objectives of this study was to test the hypothesis that intracellular accumulation of GABA improves osmotic tolerance in the unconventional yeast Candida glycerinogenes. In C. glycerinogenes, the expression of UGA4 encoding G ABA-specific permease is highly induced by hyperosmotic stress. Exogenous GABA application enhanced intracellular GABA accumulation and promoted cell growth under hyperosmotic conditions. Overexpression of the glutamate decarboxylase gene GAD1 resulted in an increased intracellular GABA and improvement in cell growth under hyperosmotic conditions. These results indicated that improving intracellular GABA accumulation of C. glycerinogenes, either through exogenous application or cellular synthesis, is available for improving the tolerance to hyperosmotic stress. We demonstrate that GABA accumulation plays an important rote in osmotic stress resistance of the unconventional yeast C. glycerinogenes.