IDENTIFICATION AND NUCLEOTIDE-SEQUENCES OF 2 SIMILAR TANDEM DIRECT REPEATS IN EPSTEIN-BARR VIRUS-DNA
IDENTIFICATION AND NUCLEOTIDE-SEQUENCES OF 2 SIMILAR TANDEM DIRECT REPEATS IN EPSTEIN-BARR VIRUS-DNA
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DOI：
10.1128/jvi.44.3.823-833.1982
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发表时间：
1982-01-01
影响因子：
5.4
通讯作者：
KIEFF, E
中科院分区：
文献类型：
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作者：
DAMBAUGH, TR;KIEFF, E
已知Epstein-Barr病毒DNA在长的独特区域的左端和右端附近具有部分同源的片段，称为DL和DR。DL和DR各自部分地由串联同向重复序列组成。DL含有11-14个重复的124个碱基对序列，命名为IR 2。DR包含. apprx。103个碱基对序列的30个直接重复，命名为IR 4。DL和DR序列对于λ具有共线性部分同源性。2.4 IR 2和IR 4右侧分别有1.5个酶对。IR 2和IR 4是相似的序列，并且部分地从共同的祖先进化而来。这两个序列都是84%的鸟嘌呤和胞嘧啶，与EB病毒IR 1和单纯疱疹病毒1型反向末端重复序列具有有限的同源性。IR 2编码在生产性感染的绒猴淋巴母细胞B 95 -8细胞中表达的丰富的2.5 β-淀粉酶持久性早期EBV RNA的一部分，但不编码在潜伏性感染的细胞中从EB病毒基因组的相邻IR 1-U2区域转录的3-β-淀粉酶EB病毒RNA的一部分。
Epstein-Barr virus DNA is known to have partially homologous segments, designated DL and DR, near the left and right ends of the long unique region. DL and DR are each partially composed of tandem direct repeat sequences. DL contains 11-14 repeats of a 124 base-pair sequence designated IR2. DR contains .apprx. 30 direct repeats of a 103 base-pair sequence designated IR4. The DL and DR sequences have colinear partial homology for .apprx. 2.4 and 1.5 kilobase pairs to the right of IR2 and IR4, respectively. IR2 and IR4 are similar sequences and evolved in part from a common ancestor. Both sequences are 84% guanine and cytosine and have limited homology to Epstein-Barr virus IR1 and to the herpes simplex virus type 1 inverted terminal repeat a sequence. IR2 encodes part of an abundant 2.5 kilobase persistent early EBV RNA expressed in productively infected marmoset lymphoblastoid B95-8 cells, but does not encode part of the 3-kilobase Epstein-Barr virus RNA which is transcribed from the adjacent IR1-U2 region of the Epstein-Barr virus genome in latently infected cells.