Stimulating effect of graphene oxide on myogenesis of C2C12 myoblasts on RGD peptide-decorated PLGA nanofiber matrices.
Stimulating effect of graphene oxide on myogenesis of C2C12 myoblasts on RGD peptide-decorated PLGA nanofiber matrices.
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DOI：
10.1186/s13036-015-0020-1
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发表时间：
2015
影响因子：
5.6
通讯作者：
Han DW
中科院分区：
文献类型：
--
作者：
Shin YC;Lee JH;Kim MJ;Hong SW;Kim B;Hyun JK;Choi YS;Park JC;Han DW
在生物医学工程领域，许多研究都集中在石墨烯和相关纳米材料的可能应用上，因为它们具有用作支架、涂层材料和递送载体的潜力。另一方面，由多种生物相容性聚合物组成的静电纺丝基质在组织工程和再生医学方面引起了极大的关注。然而，他们的组合很有趣，而且仍然具有挑战性。在本研究中，我们制作了由表面展示有RGD肽的M13噬菌体（RGD-M13噬菌体）和聚（乳酸-羟基乙酸共聚物，PLGA）组成的微球基质，并表征了它们的理化性质。此外，研究了氧化石墨烯（GO）对RGD-M13噬菌体修饰的PLGA（RGD/PLGA）载体上培养的C2 C12成肌细胞细胞行为的影响。实验结果表明，RGD/PLGA复合材料具有良好的组织工程支架的理化性能，C2 C12成肌细胞在复合材料上的生长明显增强。此外，C2 C12成肌细胞的肌源性分化被大大刺激时，他们在RGD/PLGA基质中存在的GO培养。总之，这些研究结果表明，RGD/PLGA基质和GO的组合可以用作骨骼组织工程和再生的有前途的策略。
In the field of biomedical engineering, many studies have focused on the possible applications of graphene and related nanomaterials due to their potential for use as scaffolds, coating materials and delivery carriers. On the other hand, electrospun nanofiber matrices composed of diverse biocompatible polymers have attracted tremendous attention for tissue engineering and regenerative medicine. However, their combination is intriguing and still challenging. In the present study, we fabricated nanofiber matrices composed of M13 bacteriophage with RGD peptide displayed on its surface (RGD-M13 phage) and poly(lactic-co-glycolic acid, PLGA) and characterized their physicochemical properties. In addition, the effect of graphene oxide (GO) on the cellular behaviors of C2C12 myoblasts, which were cultured on PLGA decorated with RGD-M13 phage (RGD/PLGA) nanofiber matrices, was investigated. Our results revealed that the RGD/PLGA nanofiber matrices have suitable physicochemical properties as a tissue engineering scaffold and the growth of C2C12 myoblasts were significantly enhanced on the matrices. Moreover, the myogenic differentiation of C2C12 myoblasts was substantially stimulated when they were cultured on the RGD/PLGA matrices in the presence of GO. In conclusion, these findings propose that the combination of RGD/PLGA nanofiber matrices and GO can be used as a promising strategy for skeletal tissue engineering and regeneration.