Lithography Technique for Topographical Micropatterning of Collagen-Glycosaminoglycan Membranes for Tissue Engineering Applications.
Lithography Technique for Topographical Micropatterning of Collagen-Glycosaminoglycan Membranes for Tissue Engineering Applications.
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DOI：
10.1115/1.2775937
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发表时间：
2007-09-01
期刊：
影响因子：
--
通讯作者：
Baskaran, Harihara
中科院分区：
文献类型：
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作者：
Janakiraman, Vijayakumar;Kienitz, Brian L;Baskaran, Harihara
背景技术背景：微图案化生物材料支架的适应性技术在控制细胞功能和组织工程（TE）微血管系统的发展中具有巨大的意义。在本文中，我们报告了一种技术，嵌入到胶原蛋白-糖胺聚糖（CG）膜的第一步，建立TE结构与内置的微血管的微型图案。 接近方法：通过将I型牛胶原蛋白和6-硫酸软骨素溶于乙酸中并随后真空过滤该溶液来制备CG膜。微图案化技术由三个步骤组成：使用乙酸溶液的基础基质的表面溶解，通过施加均匀压力的特征分辨率和通过戊二醛交联的特征稳定性。 结果：新技术的应用产生了CG膜的图案，空间分辨率为2-3微米。我们表明，这种图案化的矩阵是有利于附着的牛主动脉内皮细胞（BAEC的）。 结论：图案化的膜可用于开发具有内置流动通道的复杂三维TE产品，作为地形定向细胞生长的模板，或作为模型系统来研究各种微血管疾病，其中特征尺度是重要的。这项新技术是通用的;地形图案可以定制任何预定的设计与高空间分辨率和技术本身可以适用于其他支架材料。
BACKGROUND: An adaptable technique for micropatterning biomaterial scaffolds has enormous implications in controlling cell function and in the development of tissue-engineered (TE) microvasculature. In this paper, we report a technique to embed microscale patterns onto a collagen-glycosaminoglycan (CG) membrane as a first step towards the creation of TE constructs with built-in microvasculature. METHOD OF APPROACH: The CG membranes were fabricated by homogenizing a solution of Type I bovine collagen and chondroitin 6-sulfate in acetic acid and vacuum filtering the solution subsequently. The micropatterning technique consisted of three steps: surface dissolution of base matrix using acetic acid solution, feature resolution by application of uniform pressure and feature stability by glutaraldehyde crosslinking. RESULTS: Application of the new technique yielded patterns in CG membranes with a spatial resolution in the order of 2-3 microns. We show that such a patterned matrix is conducive to the attachment of bovine aortic endothelial cells (BAEC's). CONCLUSIONS: The patterned membranes can be used for the development of complex three-dimensional TE products with built-in flow channels, as templates for topographically directed cell growth, or as a model system to study various microvascular disorders where feature scales are important. The new technique is versatile; topographical patterns can be custom-made for any predetermined design with high spatial resolution and the technique itself can be adapted for use with other scaffold materials.