Calorie restriction modifies ubiquinone and COQ transcript levels in mouse tissues.
Calorie restriction modifies ubiquinone and COQ transcript levels in mouse tissues.
复制标题
热量限制会改变小鼠组织中的泛醌和 COQ 转录水平。
DOI：
10.1016/j.freeradbiomed.2011.03.024
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发表时间：
2011
影响因子：
7.4
通讯作者：
Villalba,JoséM
中科院分区：
文献类型：
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作者：
Parrado-Fernández,Cristina;López-Lluch,Guillermo;Rodríguez-Bies,Elisabet;Santa-Cruz,Sara;Navas,Plácido;Ramsey,JonJ;Villalba,JoséM
我们研究了随意喂养或热量限制的小鼠组织中泛醌 (Q)、Q 同系物比率和 mCOQ 转录物的稳态水平。以蛋白质为基础的泛醌水平最高的是肾脏和心脏，其次是肝脏、大脑和骨骼肌。肝脏和骨骼肌显示出最高的 Q9/Q10 比率，且存在显着的个体差异。心脏、肾脏，特别是大脑表现出较低的 Q9/Q10 比率和个体间变异性。在骨骼肌和心脏中，最丰富的 mCOQ 转录物是 mCOQ7，其次是 mCOQ8、mCOQ2、mPDSS2、mPDSS1 和 mCOQ3。在非肌肉组织（肝、肾和脑）中，最丰富的 mCOQ 转录物是 mCOQ2，其次是 mCOQ7、mCOQ8、mPDSS1、mPDSS2 和 mCOQ3。热量限制会增加两者骨骼肌中泛醌同系物和mPDSS2mRNA增加，但mCOQ7减少。相比之下，Q9 和大多数 mCOQ 转录本在心脏中减少。热量限制还改变了 Q9/Q10 比率，该比率在肾脏中增加，在心脏中减少，而 mPDSS1 或 mPDSS2 转录本没有改变。我们首次证明肌肉和非肌肉组织中存在 mCOQ 转录本的独特模式，并且 Q 和 COQ 基因是以组织特异性方式限制热量的目标。
We studied ubiquinone (Q), Q homologue ratio, and steady-state levels of mCOQ transcripts in tissues from mice fed ad libitum or under calorie restriction. Maximum ubiquinone levels on a protein basis were found in kidney and heart, followed by liver, brain, and skeletal muscle. Liver and skeletal muscle showed the highest Q9/Q10ratios with significant interindividual variability. Heart, kidney, and particularly brain exhibited lower Q9/Q10ratios and interindividual variability. In skeletal muscle and heart, the most abundant mCOQ transcript wasmCOQ7,followed bymCOQ8, mCOQ2, mPDSS2, mPDSS1,andmCOQ3.In nonmuscular tissues (liver, kidney, and brain) the most abundant mCOQ transcript wasmCOQ2,followed bymCOQ7, mCOQ8, mPDSS1, mPDSS2,andmCOQ3.Calorie restriction increased both ubiquinone homologues andmPDSS2mRNA in skeletal muscle, butmCOQ7was decreased. In contrast, Q9and most mCOQ transcripts were decreased in heart. Calorie restriction also modified the Q9/Q10ratio, which was increased in kidney and decreased in heart without alterations inmPDSS1ormPDSS2transcripts. We demonstrate for the first time that unique patterns of mCOQ transcripts exist in muscular and nonmuscular tissues and that Q and COQ genes are targets of calorie restriction in a tissue-specific way.