Cooperating Commensals Restore Colonization Resistance to Vancomycin-Resistant Enterococcus faecium.
Cooperating Commensals Restore Colonization Resistance to Vancomycin-Resistant Enterococcus faecium.
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DOI：
10.1016/j.chom.2017.04.002
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发表时间：
2017-05-10
影响因子：
30.3
通讯作者：
Pamer EG
中科院分区：
文献类型：
--
作者：
Caballero S;Kim S;Carter RA;Leiner IM;Sušac B;Miller L;Kim GJ;Ling L;Pamer EG
抗生素介导的微生物区系破坏和随之而来的定植抵抗力的丧失可导致万古霉素耐药肠球菌(VRE)在肠道中占据主导地位，从而导致住院患者的血液感染。清除VRE仍然是一个具有挑战性的目标，如果实现，将减少全身性VRE感染和患者之间的传播。尽管专性厌氧共生菌与对VRE的定植抗性有关，但涉及的特定细菌种类仍未确定。在这里，我们证明了一个精确定义的共生细菌联合体，包括梭状芽孢杆菌簇XIVa种Blautia Producta和Clostridium bolteae，可以恢复对VRE的定植抗性，并从小鼠的肠道中清除VRE。虽然博尔泰弧菌不直接介导VRE的清除，但它能够使B.Producta在肠道定植，从而直接抑制VRE的生长。这些发现表明，对微生物区系组成受损的个人进行治疗性或预防性给药可能会减少高度耐药病原体的患者间传播和患者内传播。万古霉素耐药肠球菌(VRE)可密集定植于肠道，引起血液感染。肠道微生物群对VRE的定植具有抵抗力。卡瓦列罗和他的同事在小鼠身上证明了Blautia Producta和Clostridium bolteae恢复了对VRE的抵抗力。给予特定的共生菌群可以重建对高度耐药病原体的定植抵抗力。
Antibiotic-mediated microbiota destruction and the consequent loss of colonization resistance can result in intestinal domination with vancomycin-resistant Enterococcus (VRE), leading to bloodstream infection in hospitalized patients. Clearance of VRE remains a challenging goal that, if achieved, would reduce systemic VRE infections and patient-to-patient transmission. Although obligate anaerobic commensal bacteria have been associated with colonization resistance to VRE, the specific bacterial species involved remain undefined. Herein we demonstrate that a precisely defined consortium of commensal bacteria containing the Clostridium cluster XIVa species Blautia producta and Clostridium bolteae restores colonization resistance against VRE and clears VRE from the intestines of mice. While C. bolteae did not directly mediate VRE clearance it enabled intestinal colonization with B. producta, which directly inhibited VRE growth. These findings suggest that therapeutic or prophylactic administration of defined bacterial consortia to individuals with compromised microbiota composition may reduce inter-patient transmission and intra-patient dissemination of highly antibiotic-resistant pathogens. Vancomycin-resistant Enterococcus (VRE) can densely colonize intestines and cause bloodstream infections. The intestinal microbiota provides resistance against VRE colonization. Caballero and colleagues demonstrate in mice that Blautia producta and Clostridium bolteae restore resistance against VRE. Administration of specific consortia of commensal bacteria can re-establish colonization resistance against highly antibiotic-resistant pathogens.