Comparative Metabolomic Analysis of the Green Microalga Chlorella sorokiniana Cultivated in the Single Culture and a Consortium with Bacteria for Wastewater Remediation
Comparative Metabolomic Analysis of the Green Microalga Chlorella sorokiniana Cultivated in the Single Culture and a Consortium with Bacteria for Wastewater Remediation
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DOI：
10.1007/s12010-017-2484-6
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发表时间：
2017-11-01
影响因子：
3
通讯作者：
Wei, Wei
中科院分区：
文献类型：
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作者：
Chen, Taojing;Zhao, Quanyu;Wei, Wei
微藻与多种细菌共培养，在废水修复中对COD、氮、磷的处理效率与单一培养相比有显著差异。为了了解其机理，本研究设计了一个对比实验，分别用单一培养的绿色微藻小球藻和与假单胞菌H4联合培养的小球藻去除营养物质。通过比较代谢组谱分析，揭示了小球藻细胞对与细菌协同生长的反应，并讨论了微藻代谢调控与营养物质降解之间的可能关系。小球藻可检测到的差异代谢物包括碳水化合物、脂肪酸、氨基酸、磷酸盐、多元醇等，对已鉴定的代谢物进行正交偏最小二乘判别分析(OPLS-DA)模型表明，小球藻的代谢受到细菌的显著影响，对应于不同的处理行为。
Co-culture of microalgae with many types of bacteria usually comes out with significant different treatment efficiencies for COD, nitrogen, and phosphorus in wastewater remediation, compared with the single culture. In order to understand the mechanism behind, a comparative experiment was designed in this study, using the green microalgae species Chlorella sorokiniana in the single culture and a consortium with a bacterium, Pseudomonas H4, for nutrient removal. Comparative metabolome profile analysis was conducted to reveal the Chlorella cell responses to the synergistic growth with the bacteria, and possible relations between the metabolic regulation of microalgae and the nutrient degradation were discussed. The detectable differential metabolites of Chlorella belonged to several classes, including carbohydrates, fatty acids, amino acids, phosphates, polyols, etc. The orthogonal partial least squares discriminant analysis (OPLS-DA) model of the identified metabolites suggests the metabolism in this alga was significantly affected by the bacteria, corresponding to different treatment behaviors.