Expression of Musashi 1, a neural RNA-binding protein, in the cochlea of young adult mice
Expression of Musashi 1, a neural RNA-binding protein, in the cochlea of young adult mice
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DOI：
10.1016/j.neulet.2003.10.036
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发表时间：
2004-01-16
影响因子：
2.5
通讯作者：
Kubo, T
中科院分区：
文献类型：
--
作者：
Murata, J;Murayama, A;Kubo, T
Musashi1 （Msi1）是一种rna结合蛋白，表达于脊椎动物中枢神经系统的神经干/祖细胞、星形胶质祖细胞和星形胶质细胞中。我们推测Msi I仅在耳蜗的部分支持细胞中表达，这些细胞在损伤后的特殊情况下可能成为毛细胞祖细胞。为此，我们利用Msi1单克隆抗体免疫组化方法研究了Msi1在年轻成年小鼠耳蜗中的表达。在Corti器官的多种支持细胞中均发现Msi1免疫染色，但在外毛细胞中未发现。虽然在内层毛细胞周围发现了一个免疫反应环，但它似乎也起源于支持细胞。我们认为Msi1在支持细胞中的广泛表达表明，这些细胞在受到损伤时可能具有成为毛细胞祖细胞的潜力，但其他一些机制严格抑制了这种能力。2003爱思唯尔爱尔兰有限公司版权所有。
Musashi1 (Msi1) is an RNA-binding protein expressed in neural stem/progenitor cells, astroglial progenitor cells and astrocytes in the vertebrate central nervous system. We hypothesized that Msi I is expressed in only some of the supporting cells in the cochlea, which could become hair cell progenitors under special circumstances after an injury. To observe this, we investigated Msi1 expression in young adult mouse cochlea by immunohistochemistry using monoclonal antibody against Msi1. Msi1 immunostaining was found in a variety of supporting cells but not in outer hair cells in the organ of Corti. Although an immunoreactive ring was found around the inner hair cells, it also seemed to originate from the supporting cells. We suppose that this wide expression of Msi1 in supporting cells indicates that those cells might have the potential to become hair cell progenitors if injured, but that some other mechanisms strictly inhibit this ability. (C) 2003 Elsevier Ireland Ltd. All rights reserved.