Surface grafting of poly (L-glutamates). 1. Synthesis and characterization
Surface grafting of poly (L-glutamates). 1. Synthesis and characterization
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DOI：
10.1021/la001769s
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发表时间：
2001-10
期刊：
影响因子：
3.9
通讯作者：
R. H. Wieringa;E. Siesling;P. Geurts;P. Werkman;E. Vorenkamp;V. Erb;M. Stamm;A. Schouten
中科院分区：
文献类型：
--
作者：
R. H. Wieringa;E. Siesling;P. Geurts;P. Werkman;E. Vorenkamp;V. Erb;M. Stamm;A. Schouten
研究了γ-氨丙基三乙氧基硅烷（APS）预处理后的硅片和石英片上谷氨酸γ-苄酯和谷氨酸γ-甲酯的N-羧基氰（NCA）开环聚合反应。FT-IR透射光谱、圆二色性测量和UV/维斯光谱证实了接枝多肽层的纯α-螺旋构象。FT-IR光谱还表明，聚合物生长的最重要的部分发生在第一个5小时的聚合。在短时间内生长的相当刚性的α-螺旋多肽的平均取向比在较长反应时间内生长的聚合物的取向更垂直于基底。对于浓度高达2.0 M，从两个NCA单体的聚合物生长显示出显着的依赖于单体浓度。单体浓度越高，螺旋结构的平均取向越垂直。
The ring-opening polymerization of N-carboxyanhydrides (NCA) of γ-benzyl l-glutamate and γ-methyl l-glutamate from (γ-aminopropyl)triethoxysilane (APS) pretreated substrates such as silicon wafers and quartz slides was investigated. FT-IR transmission spectroscopy, circular dichroism measurements, and UV/vis spectroscopy confirmed the pure α-helix conformation of the grafted polypeptide layers. FT-IR spectroscopy also showed that the most important part of the polymer growth took place in the first 5 h of the polymerization. The average orientation of the rather rigid α-helical polypeptides, grown during a short period of time, was more perpendicular with respect to the substrate than the orientation of the polymers grown over a longer reaction time. For concentrations up to 2.0 M, the polymer growth from both NCA monomers showed a pronounced dependence on the monomer concentration. Moreover, it appeared that the higher the monomer concentration, the more perpendicular the average orientation of the helic...