Identification of chromosome region and candidate genes for canary-yellow flesh (Cyf) locus in watermelon (Citrullus lanatus)
Identification of chromosome region and candidate genes for canary-yellow flesh (Cyf) locus in watermelon (Citrullus lanatus)
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DOI：
10.1016/j.plantsci.2023.111594
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发表时间：
2023-01-17
期刊：
影响因子：
5.2
通讯作者：
Luan, Feishi
中科院分区：
文献类型：
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作者：
Liu, Shi;Liu, Mengqiu;Luan, Feishi
西瓜果肉颜色的遗传控制是复杂的，仍然存在着重大的知识空白。本研究利用西瓜自交系Pi 635597与另一自交系Cam of Saskatchewan(肉色为淡黄色)杂交产生的分离群体，研究了西瓜自交系Pi 635597果肉颜色为淡黄色的遗传基础。我们发现，控制淡黄色果肉颜色的是一个显性基因，即淡黄色果肉。批量分离分析(BSA)和精细的遗传定位将Cyf基因座的范围缩小到6号染色体上79.62kb的区域，其中包含10个预测基因。两个不同果肉颜色的西瓜亲本和材料的启动子和编码区序列变异分析和基因表达分析均支持将未知蛋白基因Cla97C06G122050和五肽重复序列Cla97C06G122120预测为Cyf基因座的候选基因。分子标记辅助选择和序列比对表明，该基因座可以区分淡黄色和淡黄色的果肉。我们的结果表明，细胞色素F基因座可能与金黄色的果肉颜色和类胡萝卜素的积累水平有关。
Genetic control of fruit flesh color in watermelon is complex, and significant knowledge gaps still exist. In the present study, we investigated the genetic basis of canary-yellow flesh color in watermelon inbred line PI 635597 using a segregating population derived from a cross between PI 635597 and another inbred line, Cream of Saskatchewan (pale yellow flesh color). We showed that a single dominant gene controls the canary-yellow flesh color for the Cyf (canary-yellow flesh) trait. Bulk segregant analysis (BSA) and fine genetic mapping narrowed down the Cyf locus to a 79.62-kb region on chromosome 6, which harbors 10 predicted genes. Sequence variation analysis in the promoter and coding regions and gene expression analysis in both parental lines and selected watermelon accessions with diverse fruit flesh colors support Cla97C06G122050 (unknown protein) and Cla97C06G122120 (pentatricopeptide repeat) as predicted candidate genes for the Cyf locus. Marker-assisted selection and sequence alignment showed that the Cyf locus could differentiate canary-yellow flesh and pale -yellow flesh. Our results indicate that the Cyf locus might be responsible for canary-yellow flesh color and carotenoid accumulation levels.