The genetic structure of Beta vulgaris ssp maritima (sea beet) populations: RFLPs and isozymes show different patterns of gene flow
The genetic structure of Beta vulgaris ssp maritima (sea beet) populations: RFLPs and isozymes show different patterns of gene flow
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DOI：
10.1038/hdy.1996.138
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发表时间：
1996-09-01
期刊：
影响因子：
3.8
通讯作者：
Clarke, RT
中科院分区：
文献类型：
--
作者：
Raybould, AF;Mogg, RJ;Clarke, RT
对 12 个海甜菜群体的 9 个同工酶和 7 个 RFLP 位点的遗传变异进行了评估。两类标记之间观察到的平均杂合性、多样性指数和每个基因座的等位基因数量没有显着差异。使用七种同工酶和六种 RFLP 分析了 10 个种群的遗传结构。分别计算同工酶和 RFLP 的所有群体对之间的 F-ST 值。在岛屿模型的假设下，F-ST 值被转换为种群之间的基因流量 (Nm)。使用对数 Nm 与对数距离的回归来测试距离的隔离度。 Mantel 测试表明，对于 RFLP，Nm 随着距离的增加而显着降低，但同工酶则不然。建议均匀平衡选择可以维持同工酶基因座处群体中大致相等的等位基因频率。如果这是真的，那么同工酶可能不适合模拟中性转基因的传播。
Genetic variation in 12 populations of sea beet was assessed at nine isozyme and seven RFLP loci. Mean observed heterozygosity, diversity index and number of alleles per locus were not significantly different between the two classes of marker. The genetic structure of 10 of the populations was analysed using seven of the isozymes and six of the RFLPs. F-ST values between all pairs of populations were calculated separately for the isozymes and RFLPs. F-ST values were converted to amounts of gene flow (Nm) between populations under the assumptions of an island model. A regression of log Nm against log distance was used to test for isolation by distance. Mantel tests showed a highly significant decrease in Nm with distance for RFLPs but not for isozymes. It is suggested that uniform balancing selection may operate to maintain approximately equal allele frequencies among populations at the isozyme loci. If this is true then isozymes may be unsuitable for modelling the spread of neutral transgenes.