Angiotensin II increases lipogenesis in 3T3-L1 and human adipose cells
Angiotensin II increases lipogenesis in 3T3-L1 and human adipose cells
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DOI：
10.1210/en.138.4.1512
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发表时间：
1997-04-01
期刊：
影响因子：
4.8
通讯作者：
Moustaid, N
中科院分区：
文献类型：
--
作者：
Jones, BH;Standridge, MK;Moustaid, N
血管紧张素II（AngII）是最近显示由脂肪细胞合成和分泌的众多激素之一。虽然血管紧张素II在脂肪组织中的功能是未知的，一些研究间接表明，它可能参与控制肥胖。然而，关于血管紧张素II在脂肪细胞中的直接作用知之甚少。为了进一步研究这个问题，我们首先确定了3 T3-LI脂肪细胞中Ang II受体的类型。然后，我们使用3 T3-L1和人脂肪细胞模型测试了Ang II对脂肪细胞代谢产生直接作用的假设。我们在这里报告，血管紧张素IT显着增加甘油三酯含量和两个嘿脂肪生成酶（脂肪酸合成酶，FAS和甘油-3-磷酸脱氢酶，GPDH）在3 T3-L1脂肪细胞的活性，这些作用是通过2型血管紧张素II受体介导的。我们还报告，血管紧张素II在人脂肪细胞维持在原代培养中发挥类似的作用，最后，我们证明，血管紧张素II增加FAS和ob基因在3 T3-L1和人脂肪细胞的转录速率。这些结果表明，血管紧张素Ⅱ可能参与控制肥胖，通过调节脂肪细胞中的脂质合成和储存。
Angiotensin II (Ang II) is one of numerous hormones recently shown to be synthesized and secreted by adipose cells. Although the function of Ang II in adipose tissue is unknown, several studies indirectly suggest that it may be involved in control of adiposity. Little is known, however, about direct actions of Ang II in adipose cells. To further investigate this issue, we first characterized the type of Ang II receptors in 3T3-LI adipocytes. We then tested the hypothesis that Ang II exerted direct actions on adipocyte metabolism using both 3T3-L1 and human adipocyte models. We report here that Ang IT significantly increased triglyceride content and the activities of two hey lipogenic enzymes (fatty acid synthase, FAS and glycerol-3-phosphate dehydrogenase, GPDH) in 3T3-L1 adipocytes, and that these effects were mediated through the type-2 Ang II receptor. We also report that Ang II exerted similar effects in human adipose cells maintained in primary culture, Finally, we demonstrate that Ang II increased the transcription rate of the FAS and ob genes in 3T3-L1 and human adipose cells. These results indicate that Ang II may be involved in control of adiposity through regulation of lipid synthesis and storage in adipocytes.