In Vitro Quantitative Autoradiography of [3H]‐L‐Deprenyl and [3H]‐PK 11195 Binding Sites in Human Epileptic Hippocampus
In Vitro Quantitative Autoradiography of [3H]‐L‐Deprenyl and [3H]‐PK 11195 Binding Sites in Human Epileptic Hippocampus
复制标题
人癫痫海马中 [3H]-L-Deprenyl 和 [3H]-PK 11195 结合位点的体外定量放射自显影
作者：
E. Kumlien;P. Hilton;B. Spännare;P. Gillberg
摘要：以[3H]L -去戊烯基和[3H]PK 11195两种神经胶质层标记物为配体，采用定量放射自成像技术研究了单胺氧化酶B （MAO‐B）和外周苯二氮卓结合位点（ψ3位点）的分布。研究了7例因难治性复杂部分性癫痫而行前颞叶切除的患者的硬化海马，并与3例非癫痫对照的死后海马进行了比较。在癫痫患者中，L -去戊烯基与PK 11195的结合程度显著提高。两种配体结合的增加与神经元损失的程度相关，但只有PK 11195与胶质瘤的程度相关。我们的结论是，这两种配体都可以为定量神经胶质瘤在病理状态（如癫痫）的程度提供有用的标记。它们可能适用于未来的正电子发射断层扫描（PET）的体内研究。
Summary: Distribution of the enzyme monoamine oxidase B (MAO‐B) and the peripheral benzodiazepine binding site (ψ3 site) was studied by quantitative autoradiography using [3H]L‐deprenyl and [3H]PK 11195, two tentative glial markers, as ligands. Sclerotic hippocampus from seven patients who had had anterotemporal lobe resection because of intractable complex partial epilepsy were investigated and compared with postmortem hippocampus from three nonepileptic controls. A significantly higher degree of L‐deprenyl and PK 11195 binding was observed in the epileptic cases. The increased binding of both ligands correlated to extent of neuronal loss, but only PK 11195 showed correlation to degree of gliosis. We conclude that both ligands could provide useful markers for quantitating the degree of gliosis in pathologic states such as epilepsy. They may be applicable in future in vivo studies with positron emission tomography (PET).