Occurrence of changes in human rotavirus serotypes with concurrent changes in genomic RNA electropherotypes
Occurrence of changes in human rotavirus serotypes with concurrent changes in genomic RNA electropherotypes
复制标题
人类轮状病毒血清型发生变化，同时基因组 RNA 电泳型发生变化
DOI：
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发表时间：
1988
影响因子：
9.4
通讯作者：
O. Nakagomi
中科院分区：
文献类型：
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作者：
T. Nakagomi;K. Akatani;N. Ikegami;N. Katsushima;O. Nakagomi
为了研究人类轮状病毒株的血清型和遗传多样性，我们通过血清型特异性单克隆抗体的酶联免疫吸附测定和分段RNA基因组的聚丙烯酰胺凝胶电泳分别测试了513份和519份粪便轮状病毒样本。在 513 份标本中，375 份被分型为血清型 1 (47.3%)、血清型 2 (2.9%)、血清型 3 (2.9%) 或血清型 4 (17.7%)。此外，在 1.6% 的测试样本中发现了一种推定的新人类血清型，本文暂称为 X 血清型。其余 138 个样本 (26.9%) 无法打字。根据地理位置和观察期，观察到循环血清型的相对频率存在相当大的变化。在 519 份测试样本中，有 481 份显示出轮状病毒 RNA。其中，415 种被分类为 33 种电泳型，其中许多很少检测到，并且仅限于单一流行病。对 291 个具有电泳图型和血清型的样本的分析清楚地表明，给定的 RNA 模式总是对应于特定的血清型。在属于四种先前建立的血清型的菌株中，类似地观察到血清型内电泳型的异质性。本研究获得的结果表明，主要中和抗原的抗原变化总是与基因组RNA电泳型的变化同时发生。另一方面，不能从 RNA 电泳型的变化来预测血清型的变化。
To investigate the serotypic and genetic diversity of human rotavirus strains, we have tested 513 and 519 fecal rotavirus specimens, respectively, by an enzyme-linked immunosorbent assay with serotype-specific monoclonal antibodies and by polyacrylamide gel electrophoresis of the segmented RNA genome. Of the 513 specimens, 375 were typed as serotype 1 (47.3%), serotype 2 (2.9%), serotype 3 (2.9%), or serotype 4 (17.7%). In addition, a presumptive new human serotype, tentatively referred to as serotype X in this paper, was found in 1.6% of the specimens tested. The remaining 138 specimens (26.9%) were untypeable. Considerable variation in relative frequency of circulating serotypes was observed with respect to geographic locations and observation periods. Rotavirus RNAs were visualized in 481 of 519 specimens tested. Of these, 415 were typed as 33 electropherotypes, many of which were infrequently detected and were restricted to single epidemics. Analysis of the 291 specimens whose electropherotypes and serotypes were available indicated clearly that a given RNA pattern always corresponded to a particular serotype. Heterogeneity of electropherotypes within a serotype was similarly observed in strains belonging to the four previously established serotypes. The results obtained in this study indicated that antigenic changes on the major neutralization antigen occurred always with concurrent changes of genomic RNA electropherotypes. On the other hand, serotypic changes could not be predicted from the changes in RNA electropherotypes.