ERYTHROPOIETIN EFFECT ON SENSORIMOTOR RECOVERY AFTER CONTUSIVE SPINAL CORD INJURY: AN ELECTROPHYSIOLOGICAL STUDY IN RATS
ERYTHROPOIETIN EFFECT ON SENSORIMOTOR RECOVERY AFTER CONTUSIVE SPINAL CORD INJURY: AN ELECTROPHYSIOLOGICAL STUDY IN RATS
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DOI：
10.1016/j.neuroscience.2012.05.041
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发表时间：
2012-09-06
期刊：
影响因子：
3.3
通讯作者：
Gorio, A.
中科院分区：
文献类型：
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作者：
Cerri, G.;Montagna, M.;Gorio, A.
脊髓损伤（SCI）是一种使人衰弱的临床病症，其特征是复杂的神经功能障碍。大鼠实验显示，挫伤性 SCI 后急性施用重组人促红细胞生成素 (rhEPO) 可改善后肢运动功能的恢复，根据运动 BBB（Basso、Beattie、Bresnahan）量表测量。该量表评估整体运动活动，但没有测试 rhEPO 诱导的运动恢复是否是由于感觉和/或运动通路的并行恢复所致。本研究的目的是利用电生理学测试来评估，在挫伤性 SCI 大鼠模型中，使用 rhEPO (n = 25) 与盐溶液 (n = 25) 治疗的动物在损伤后 2、5、7、11 和 30 天时穿过受损脊髓的上升和下降通路的传输。在脊髓中记录由上皮层刺激引起的运动电位，在皮质水平记录由脊髓刺激引起的感觉诱发电位。在相同的动物中，对病变尾部的脊柱节段进行 BBB 评分和免疫细胞化学评估。在rhEPO治疗的动物中，结果表明，相对于盐水对照，通过备用脊髓通路（推测是通过网状脊髓系统）的感觉和运动传递都有更好的总体改善。这种改进在相对较早的时期最为突出。总体而言，这些特征显示与 BBB 评分中观察到的变化平行的时间过程，表明 EPO 介导的备用脊髓通路可能有助于传输的改善，而传输的改善反过来又可能导致运动功能的恢复。 (c) 2012 年国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
Spinal cord injury (SCI) is a debilitating clinical condition, characterized by a complex of neurological dysfunctions. It has been shown in rats that the acute administration of recombinant human erythropoietin (rhEPO) following a contusive SCI improves the recovery of hindlimb motor function, as measured with the locomotor BBB (Basso, Beattie, Bresnahan) scale. This scale evaluates overall locomotor activity, without testing whether the rhEPO-induced motor recovery is due to a parallel recovery of sensory and/or motor pathways. Aim of the present study was to utilize an electrophysiological test to evaluate, in a rat model of contusive SCI, the transmission of both ascending and descending pathways across the damaged cord at 2, 5, 7, 11, and 30 days after lesion, in animals treated with rhEPO (n = 25) vs saline solution (n = 25). Motor potentials evoked by epicortical stimulation were recorded in the spinal cord, and sensory-evoked potentials evoked by spinal stimulation were recorded at the cortical level. In the same animals BBB score and immunocytochemical evaluation of the spinal segments caudal to the lesion were performed. In rhEPO-treated animals results show a better general improvement both in sensory and motor transmission through spared spinal pathways, supposedly via the reticulo-spinal system, with respect to saline controls. This improvement is most prominent at relatively early times. Overall these features show a parallel time course to the changes observed in BBB score, suggesting that EPO-mediated spared spinal cord pathways might contribute to the improvement in transmission which, in turn, might be responsible for the recovery of locomotor function. (c) 2012 IBRO. Published by Elsevier Ltd. All rights reserved.